Generation of Ppp2Ca and Ppp2Cb conditional null alleles in mouse
Generation of Ppp2Ca and Ppp2Cb conditional null alleles in mouse
复制标题
DOI：
10.1002/dvg.20815
复制
发表时间：
2012-05-01
期刊：
影响因子：
1.5
通讯作者：
Gao, Xiang
中科院分区：
文献类型：
--
作者：
Gu, Pengyu;Qi, Xin;Gao, Xiang
蛋白磷酸酶2A（PP 2A）是一种最丰富的丝氨酸/苏氨酸磷酸酶，在胚胎发育和人类疾病中起着关键作用。PP 2A的催化亚基有两种异构体，Ppp 2ca和Ppp 2cb。Ppp 2ca的突变导致E6.5的早期胚胎死亡，阻碍了PP 2A在此阶段之后的功能研究。我们通过在Ppp 2ca的外显子3至5和Ppp 2cb的外显子3两侧插入loxP位点来产生Ppp 2ca和Ppp 2cb的条件无效等位基因。Ppp 2cafl/fl小鼠未显示任何可见的表型。纯合子突变体中，铬介导的切除导致全球删除Ppp 2CA显示胚胎致死性和发育缺陷类似于以前报道的。Ppp2cb？/？通过相同策略产生的小鼠没有表现出任何明显的形态学或生理学缺陷。这些小鼠品系可以作为重要的遗传工具，以空间或时间特异性的方式研究PP 2A在发育和疾病过程中的作用。Genesis 50：429436，2012. (C)2011 Wiley Periodicals，Inc.
Protein phosphatase 2A (PP2A) is one of the most abundant serine/threonine phosphatases, with a critical role in embryonic development and human disease. There are two isoforms of the catalytic subunit of PP2A, Ppp2ca and Ppp2cb. Null mutation of Ppp2ca leads to early embryonic lethality at E6.5, hindering functional study of PP2A beyond this stage. We generated conditional null alleles of Ppp2ca and Ppp2cb by flanking with loxP sites exons 3 to 5 of Ppp2ca and exon 3 of Ppp2cb. Ppp2cafl/fl mice did not display any visible phenotype. Homozygous mutants in which Cre-mediated excision resulted in global deletion of Ppp2ca displayed embryonic lethality and developmental defects similar to those previously reported. Ppp2cb?/? mice generated by the same strategy did not display any obvious morphological or physiological defects. These mouse strains can serve as important genetic tools to study the roles of PP2A during development and disease in a spatial- or temporal-specific manner. genesis 50:429436, 2012. (C) 2011 Wiley Periodicals, Inc.