Gas Chromatography-Mass Spectrometry Analysis of Human Exhaled Volatile Organic Compounds via Wearable Facemask Microextraction Sampling
Gas Chromatography-Mass Spectrometry Analysis of Human Exhaled Volatile Organic Compounds via Wearable Facemask Microextraction Sampling
复制标题
可穿戴面罩微萃取取样人体呼出挥发性有机物的气相色谱-质谱分析
DOI：
10.19756/j.issn.0253-3820.210828
复制
发表时间：
2022-03-01
影响因子：
1.2
通讯作者：
Hu Bin
中科院分区：
文献类型：
--
作者：
Chen Wei-Ni;Yuan Zi-Cheng;Hu Bin
人类呼出的气体含有大量与人类行为和健康状态相关的挥发性代谢物。本研究将固相微萃取（SPME）纤维直接插入面罩中，形成一种新型的面罩式微萃取装置，用于人体呼出气中挥发性有机化合物（VOCs）的萃取。采样结束后，将固相微萃取纤维与不同的气相色谱-质谱联用仪（GC-MS）联用，包括常规GC-MS、便携式GC-MS和综合二维GC-MS，对提取的挥发性有机物进行进一步鉴定，并对不同的固相微萃取纤维和采样时间进行比较。通过比较口罩呼出气中VOCs的背景信号，探讨了食物摄入和口腔护理对人体呼出气中VOCs组成及变化的影响。研究了呼出的外源性VOCs的可检测性和持久性。结果表明，面罩微萃取采样与GC-MS联用技术可有效检测呼出气中大量内源性和外源性VOCs。面罩式微萃取装置具有安全、简单、取样方便、适应性强等特点，可与多种GC-MS方法联用，便携式GC-MS可在数分钟内快速筛查，而GC x GC-MS可对生物标志物的发现进行全面分析。这项工作为研究人类健康和口腔疾病提供了一种新的有前途的策略。
Human exhaled breath contains a large number of volatile metabolites relating to human behavior and health states. In this study, solid phase microextraction ( SPME) fibers were directly inserted into a facemask ( SPME-in-facemask) to form a new facemask microextraction device for extracting human exhaled volatile organic compounds ( VOCs). After sampling, SPME fibers were then coupled with different gas chromatography-mass spectrometers ( GC-MS) for further identification of extracted VOCs, including conventional GC-MS, portable GC-MS and comprehensive two-dimensional GC-MS. Different SPME fibers and sampling time were investigated and compared. By comparing the background signals of face mask human exhaled VOCs, the effects of food intake and oral care on the compositions and changes of human exhaled VOCs were explored. The detectability and persistence of exhaled exogenous VOCs were investigated. The results showed that the facemask microextraction sampling coupled with GC-MS could be an efficient analytical tool for detecting a large amount of exhaled endogenous and exogenous VOCs. With the unique features of the facemask microextraction device, including safety, simplicity, convenient sampling, and strong adaptability to couple with different GC-MS methods in which portable GC-MS could provide a fast screening within minutes, while GC x GC-MS could give a comprehensive analysis for biomarker discovery. This work presented a novel promising strategy for investigating human health and oral diseases.