Predicting the prognosis in patients with sepsis by a pyroptosis-related gene signature.
Predicting the prognosis in patients with sepsis by a pyroptosis-related gene signature.
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DOI：
10.3389/fimmu.2022.1110602
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发表时间：
2022
影响因子：
7.3
通讯作者：
中科院分区：
文献类型：
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作者：
败血症仍然是一种危及生命的疾病，死亡率很高，每年在全世界造成数百万人死亡。许多研究表明，焦亡在脓毒症的发生和发展中起着重要作用。然而，焦热相关基因在脓毒症中的潜在预后和诊断价值尚不清楚。GSE65682和GSE95233数据集来源于Gene Expression Omnibus （GEO）数据库，相关基因来源于前人文献和Molecular Signature database。采用单因素cox分析和最小绝对收缩和选择算子（LASSO） cox回归分析，选择与预后差异表达的热死相关基因，构建预后风险评分。通过功能分析和免疫浸润分析，探讨脓毒症患者的生物学特性和免疫细胞富集情况。然后分析热死相关基因与免疫细胞的相关性，并利用受者工作特征曲线评价所选基因的诊断价值。在脓毒症患者和健康人之间共鉴定出16个与热休克相关的差异表达基因。建立了基于6个基因（GZMB、CHMP7、NLRP1、MYD88、ELANE和AIM2）的预后风险评分。根据风险评分将脓毒症患者分为低危组和高危组，低危组患者预后较好。功能富集分析发现，nod样受体信号通路、造血细胞谱系和其他免疫相关通路富集。免疫浸润分析显示，一些先天性和适应性免疫细胞在低、高危人群之间存在显著差异，相关分析显示6个基因均与中性粒细胞显著相关。6个基因中的4个（GZMB、CHMP7、NLRP1和AIM2）在脓毒症诊断中也具有潜在的诊断价值。我们开发并验证了一种新的基于6个焦热相关基因的脓毒症预后预测风险评分。6个基因中的4个在败血症诊断中也具有潜在的诊断价值。
Sepsis remains a life-threatening disease with a high mortality rate that causes millions of deaths worldwide every year. Many studies have suggested that pyroptosis plays an important role in the development and progression of sepsis. However, the potential prognostic and diagnostic value of pyroptosis-related genes in sepsis remains unknown. The GSE65682 and GSE95233 datasets were obtained from Gene Expression Omnibus (GEO) database and pyroptosis-related genes were obtained from previous literature and Molecular Signature Database. Univariate cox analysis and least absolute shrinkage and selection operator (LASSO) cox regression analysis were used to select prognostic differentially expressed pyroptosis-related genes and constructed a prognostic risk score. Functional analysis and immune infiltration analysis were used to investigate the biological characteristics and immune cell enrichment in sepsis patients who were classified as low- or high-risk based on their risk score. Then the correlation between pyroptosis-related genes and immune cells was analyzed and the diagnostic value of the selected genes was assessed using the receiver operating characteristic curve. A total of 16 pyroptosis-related differentially expressed genes were identified between sepsis patients and healthy individuals. A six-gene-based (GZMB, CHMP7, NLRP1, MYD88, ELANE, and AIM2) prognostic risk score was developed. Based on the risk score, sepsis patients were divided into low- and high-risk groups, and patients in the low-risk group had a better prognosis. Functional enrichment analysis found that NOD-like receptor signaling pathway, hematopoietic cell lineage, and other immune-related pathways were enriched. Immune infiltration analysis showed that some innate and adaptive immune cells were significantly different between low- and high-risk groups, and correlation analysis revealed that all six genes were significantly correlated with neutrophils. Four out of six genes (GZMB, CHMP7, NLRP1, and AIM2) also have potential diagnostic value in sepsis diagnosis. We developed and validated a novel prognostic predictive risk score for sepsis based on six pyroptosis-related genes. Four out of the six genes also have potential diagnostic value in sepsis diagnosis.
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影响因子：
4.6
作者：
AbdAllah NB;Toraih EA;Al Ageeli E;Elhagrasy H;Gouda NS;Fawzy MS;Helal GM
通讯作者：
Helal GM
影响因子：
64.8
作者：
Shi, Jianjin;Zhao, Yue;Shao, Feng
通讯作者：
Shao, Feng
DOI：
10.1016/j.apsb.2021.02.006
发表时间：
2021-09
期刊：
Acta pharmaceutica Sinica. B
影响因子：
--
作者：
Burdette BE;Esparza AN;Zhu H;Wang S
通讯作者：
Wang S
影响因子：
6.8
作者：
Zabihi M;Floris DL;Kia SM;Wolfers T;Tillmann J;Arenas AL;Moessnang C;Banaschewski T;Holt R;Baron-Cohen S;Loth E;Charman T;Bourgeron T;Murphy D;Ecker C;Buitelaar JK;Beckmann CF;Marquand A;EU-AIMS LEAP Group
通讯作者：
EU-AIMS LEAP Group
影响因子：
3.7
作者：
通讯作者：
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