Needle-type ultra micro silver/silver chloride reference electrode for use in micro-electrochemistry
Needle-type ultra micro silver/silver chloride reference electrode for use in micro-electrochemistry
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DOI：
10.2116/analsci.21.907
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发表时间：
2005-08-01
影响因子：
1.6
通讯作者：
Furukawa, M
中科院分区：
文献类型：
--
作者：
Kitade, T;Kitamura, K;Furukawa, M
构建了针型超微型银/银参比电极，其具有外径和内径分别为1.0 μ m和0.5 ± 0.2 μ m的微毛细管。该微型参比电极可以粘贴在活细胞内，适用于电化学扫描隧道显微镜的电化学电池或mu-TAS的检测部等非常小的环境。用含有3.33mol/L氯化钾的琼脂凝胶作为盐桥填充微毛细管，覆盖银丝裸露部分，并对测量池和引线进行电磁屏蔽，可获得良好的微参比电极电位稳定性和重复性。该电极在使用含有银的3.3 mol/L氯化钾水溶液作为内部电解质溶液制造后显示出稳定的电位7天。该电极在pH 5 - 9的测试溶液中显示出恒定的电极电位和优异的稳定性。在标准pH缓冲溶液中，市售pH玻璃电极与微参比电极的电极电位符合能斯特方程。
A needle-type ultra micro silver/silver chloride reference electrode having a micro capillary with outer and inner diameters of 1.0 mu m and 0.5 +/- 0.2 mu m, respectively, was constructed. This micro reference electrode can be stuck into a living cell, and is applicable to use in very small environments, such as an electrochemical cell of an electrochemical scanning tunneling microscope or the detection portion of a mu-TAS. Excellent stability and repeatability of the micro reference electrode potential could be obtained by filling the micro capillary with agar gel containing 3.33 mol/L potassium chloride as a salt bridge, by covering the bare part of the silver wire on which silver chloride was deposited, and by electromagnetic shielding of the measurement cell and wire lead from the electromagnetic waves. The electrode showed stable potential for 7 days after its fabrication using 3.3 mol/L potassium chloride aqueous solution containing silver chloride as an internal electrolyte solution. The electrode exhibited constant electrode potential and excellent stability in test solutions of pH 5 - 9. The electrode potential of a commercial pH glass electrode measured against the micro reference electrode in standard pH buffer solutions was in good accordance with the Nernst equation.