Virulence genes and antimicrobial susceptibility in Pasteurella multocida isolates from calves
Virulence genes and antimicrobial susceptibility in Pasteurella multocida isolates from calves
复制标题
DOI：
10.1016/j.vetmic.2013.09.029
复制
发表时间：
2013-12-27
影响因子：
3.3
通讯作者：
Mikami, O.
中科院分区：
文献类型：
--
作者：
Katsuda, K.;Hoshinoo, K.;Mikami, O.
对来自临床健康和患病犊牛的总共 378 株多杀性巴氏杆菌分离株进行了对 9 种抗菌药物的敏感性特征，并通过 PCR 筛选了抗菌药物耐药基因和 22 种毒力相关基因（包括荚膜生物合成基因）的存在。 在 378 株分离株中，102 株（27.0%）对 9 种测试的抗菌药物中的至少一种具有耐药性。对土霉素的耐药性（21.7%）是分离株中最常见的表型。 tet(H) 基因是检测到的主要决定因素。对甲砜霉素、氨苄青霉素、卡那霉素和氟苯尼考的耐药率分别为13.2%、5.8%、9.0%和0.5%。头孢唑林、头孢噻呋、头孢喹肟和恩诺沙星是有效的抗菌药物，在调查过程中没有出现耐药菌株。大多数分离株被鉴定为荚膜A型，只有6.3%属于荚膜D型，没有鉴定出其他荚膜类型。四个毒力相关基因（pfha、tadpA、tbpA 和 HAS）与荚膜类型相关，三个（pfhA、tbpA 和 hgbB）与动物的疾病状态相关。这些毒力基因已被认为是流行病学标记，并假设与牛的疾病结果具有很强的正相关性。 (C) 2013 Elsevier B.V. 保留所有权利。
A total of 378 isolates of Pasteurella multocida from clinically healthy and diseased calves were characterised for their susceptibility to 9 antimicrobial agents and screened by PCR for the presence of antimicrobial resistance genes and 22 genes virulence-associated, including capsule biosynthesis genes.Of the 378 isolates, 102 (27.0%) were resistant to at least one of the 9 tested antimicrobial agents. Resistance to oxytetracycline (21.7%) was the most frequently observed phenotype among the isolates. The tet(H) gene were the primary determinant detected. The resistance rates for thiamphenicol, ampicillin, kanamycin and florfenicol were 13.2%, 5.8%, 9.0% and 0.5%, respectively. Cefazolin, ceftiofur, cefquinome and enrofloxacin were effective antimicrobial agents, with no resistant isolates emerging over the course of the investigation.Most isolates were identified as capsular type A, only 6.3% belonged to capsular type D and no other capsular type was identified. Four of the virulence-associated genes (pfha, tadpA, tbpA and HAS) exhibited associations to the capsular type, and three (pfhA, tbpA and hgbB) were associated with the disease status of the animals. These virulence genes have been considered as epidemiological markers and are hypothesised to have a strong positive association with the outcome of disease in cattle. (C) 2013 Elsevier B.V. All rights reserved.