Exploring biomolecular interactions by single-molecule fluorescence
Exploring biomolecular interactions by single-molecule fluorescence
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DOI：
10.1016/j.trac.2007.09.003
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发表时间：
2007-10-01
影响因子：
13.1
通讯作者：
Reif, Randall D.
中科院分区：
文献类型：
--
作者：
Pappas, Dimitri;Burrows, Sean M.;Reif, Randall D.
近年来，单分子检测已经从专注于检测单个分子发展到检测各种系统中的分子相互作用。本文综述了单分子荧光检测在生物分子相互作用方面的最新进展。我们描述了单分子检测仪器以及使用的几个重要工具（例如，各向异性和共振能量转移）。我们还回顾了单分子检测在各种生物分子相互作用中的最新应用。(c) 2007 Elsevier Ltd.版权所有。
In recent years, single-molecule detection has evolved from focusing on detecting a single molecule to examining molecular interactions in various systems. This review discusses the recent progress of single-molecule-fluorescence detection with respect to biomolecular interactions. We describe single-molecule-detection instrumentation along with several of the important tools that are used (e.g., anisotropy and resonance-energy transfer). We also review recent applications of single-molecule detection to various types of biomolecular interactions. (c) 2007 Elsevier Ltd. All rights reserved.