Morphology of Rat Testis Preserved in Three Different Fixatives
Morphology of Rat Testis Preserved in Three Different Fixatives
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DOI：
10.1007/s11596-011-0247-0
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发表时间：
2011-04-01
影响因子：
--
通讯作者：
Zhang, Huiping
中科院分区：
文献类型：
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作者：
Tu, Lihui;Yu, Lili;Zhang, Huiping
睾丸组织学检查对评估精子发生和睾丸功能很重要。改良Davidson液（Modified Davidson's fluid，MDF）是布因液（Bouin's fluid，BF）的一种上级替代液。此外，4%多聚甲醛（PFA）已被普遍用于固定睾丸，方便。我们使用苏木精和伊红（HE）染色切片比较了固定在4% PFA、MDF或BF中的大鼠睾丸的形态学。4%PFA固定后，生精上皮细胞间出现明显的收缩伪影。在BF中固定的睾丸的中心区域也观察到收缩伪影。尽管在生殖细胞之间的曲细精管中可以观察到少量，但使用mdF不会导致曲细精管之间的收缩伪影。与4% PFA相比，在MDF和BF中固定的睾丸中细胞核细节的清晰度更好。我们的研究表明，与4% PFA和BF相比，在MDF中固定大鼠睾丸提供了更好的形态学细节。
Histopathological examination of testes is important in assessing spermatogenesis and testicular function. Modified Davidson's fluid (mDF) has been proposed as a superior substitute for Bouin's fluid (BF) for fixation of adult animal testes. Besides, 4% paraformaldehyde (PFA) has been commonly used to fix testes with convenience. We compared the morphology of the rat testis fixed in 4% PFA, mDF, or BF using hematoxylin and eosin (HE)-stained sections. Fixation in 4% PFA resulted in obvious tissue shrinkage artifacts, especially between seminiferous epithelium cells. Shrinkage artifacts were also observed in the central area of the testes fixed in BF. Use of mDF did not cause shrinkage artifacts between seminiferous tubules, though a small amount can be observed in seminiferous tubules between germ cells. Clarity of nuclear detail in testes fixed in mDF and BF is better compared to 4% PFA. Our study demonstrated that fixation in mDF provided better morphologic details in the rat testis as compared with 4% PFA and BF.