A high-density genetic linkage map of bronze loquat based on SSR and RAPD markers
A high-density genetic linkage map of bronze loquat based on SSR and RAPD markers
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DOI：
10.1007/s11295-016-1040-9
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发表时间：
2016-08-01
影响因子：
2.4
通讯作者：
Yamamoto, Toshiya
中科院分区：
文献类型：
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作者：
Fukuda, Shinji;Ishimoto, Keiichiro;Yamamoto, Toshiya
利用枇杷×青铜枇杷（Eriobotryadeflexa）三交组合，利用SSR和RAPD标记构建了青铜枇杷的高密度遗传连锁图谱。本研究中使用的SSR位点的位置先前确定的参考地图上的梨（梨属）。和苹果（Malus spp.）。青铜枇杷（“台湾枇杷1号”）的遗传图谱由308个位点组成，包括167个SSR（枇杷8个，梨57个，苹果102个），140个RAPD标记，以及位于19个连锁群上的枇杷溃疡病抗性基因Pse-a，遗传距离为1036 cM。利用至少两个梨或苹果SSR标记，几乎所有的枇杷连锁群都与梨共有图谱进行了比对，说明枇杷与梨、枇杷与苹果之间的SSR位点位置和连锁关系都很保守。构建的图谱可用于确定感兴趣的基因和数量性状基因座的位置，并分析蔷薇科绣线菊亚科派中的基因组同线性。
We constructed a high-density genetic linkage map of bronze loquat (Eriobotrya deflexa) by using a three-way cross of loquat (Eriobotrya japonica) x (loquat x bronze loquat) and simple sequence repeat (SSR) and random amplified polymorphic DNA (RAPD) markers. The positions of the SSR loci used in this study were previously identified on reference maps of pears (Pyrus spp.) and apples (Malus spp.). The map of bronze loquat ('Taiwan loquat No. 1') consisted of 308 loci including 167 SSRs (8 loquat, 57 pear, and 102 apple SSRs), 140 RAPDs, and the loquat canker resistance gene Pse-a on 19 linkage groups covering a genetic distance of 1036 cM. Almost all loquat linkage groups were aligned to the pear consensus map by using at least two pear or apple SSRs, suggesting that positions and linkages of SSR loci were well conserved between loquat and pear and between loquat and apple. The constructed map may be used to determine the location of genes and quantitative trait loci of interest and to analyze genome synteny in the tribe Pyreae, subfamily Spiraeoideae of the family Rosaceae.