ADHESION OF MACROCONIDIA TO THE PLANT-SURFACE AND VIRULENCE OF NECTRIA-HAEMATOCOCCA
ADHESION OF MACROCONIDIA TO THE PLANT-SURFACE AND VIRULENCE OF NECTRIA-HAEMATOCOCCA
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DOI：
10.1128/aem.56.12.3772-3778.1990
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发表时间：
1990-12-01
影响因子：
4.4
通讯作者：
EPSTEIN, L
中科院分区：
文献类型：
--
作者：
JONES, MJ;EPSTEIN, L
为了研究葫芦科病原体 Nectria haematococca（无性型、Fusarium solani f. sp. cucurbitae）的孢子附着，分离了具有附着缺陷的大分生孢子的突变体。用N-甲基-N''-硝基-N-亚硝基胍处理后选择粘附缺陷突变体，然后重复富集不附着于聚苯乙烯的大分生孢子。两个独立衍生的突变体产生的大分生孢子与聚苯乙烯和西葫芦果实的附着力减少了约 50%。当将大分生孢子接种到受伤的西葫芦果实中时，粘附缺陷突变体与野生型菌株一样具有毒性。然而，在将大分生孢子沉积在未受伤的西葫芦表面的疾病测定中，突变体的毒性低于野生型。因此，红球藻大分生孢子对其宿主表面的粘附似乎是一种毒力因子。
To study spore attachment of the cucurbit pathogen Nectria haematococca (anamorph, Fusarium solani f. sp. cucurbitae), mutants with adhesion-deficient macroconidia were isolated. The adhesion-deficient mutants were selected after treatment with N-methyl-N''-nitro-N-nitrosoguanidine followed by repeated enrichment for macroconidia which did not attach to polystyrene. Two independently derived mutants produced macroconidia with an approximately 50% reduction in attachment to polystyrene and to zucchini fruits. When macroconidia were inoculated into wounded zucchini fruits, the adhesion-deficient mutants were as virulent as the wild-type strain. However, in disease assays in which macroconidia were deposited onto the surface of unwounded zucchini, the mutants were less virulent than the wild type. Thus, adhesion of N. haematococca macroconidia to its host surface appears to be a virulence factor.