Oceanic 18S rDNA sequences from picoplankton reveal unsuspected eukaryotic diversity
Oceanic 18S rDNA sequences from picoplankton reveal unsuspected eukaryotic diversity
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DOI：
10.1038/35054541
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发表时间：
2001-02-01
期刊：
影响因子：
64.8
通讯作者：
Vaulot, D
中科院分区：
文献类型：
--
作者：
Moon-van der Staay, SY;De Wachter, R;Vaulot, D
皮氏细胞直径小于3 μ m是主要的贡献者都初级生产和生物量在开放的海洋区域（1，2）。然而，与原核生物相比（3），微微型浮游生物的真核成分仍然知之甚少。最近发现的新真核藻类类群的基础上微微浮游生物文化（4，5）表明存在许多未被发现的类群。基于表型标准的传统方法在描述微型浮游生物组成方面存在局限性，因为它们的尺寸很小，缺乏独特的分类特征（6）。在这里，我们分析了35个小亚基（18S）核糖体RNA基因的全序列，该方法在原核生物中非常成功（7），但到目前为止很少应用于真核生物（8），这些核糖体RNA基因来自赤道太平洋75分钟深处收集的微微真核生物组合，并表明微微真核生物具有高度多样性。大多数序列是以前未知的，但仍然可以分配到重要的海洋门，包括prasinophytes，haptophytes，甲藻，stratifiles，choanoflagellates和刺。我们还发现了一个新的谱系，与腰鞭毛虫密切相关，以前没有描述过。
Picoplankton-cells with a diameter of less than 3 mum-are the dominant contributors to both primary production and biomass in open oceanic regions(1,2). However, compared with the prokaryotes(3), the eukaryotic component of picoplankton is still poorly known. Recent discoveries of new eukaryotic algal taxa based on picoplankton cultures(4,5) suggest the existence of many undiscovered taxa. Conventional approaches based on phenotypic criteria have limitations in depicting picoplankton composition due to their tiny size and lack of distinctive taxonomic characters(6). Here we analyse, using an approach that has been very successful for prokaryotes(7) but has so far seldom been applied to eukaryotes(8), 35 full sequences of the small-subunit (18S) ribosomal RNA gene derived from a picoplanktonic assemblage collected at a depth of 75 min the equatorial Pacific Ocean, and show that there is a high diversity of picoeukaryotes. Most of the sequences were previously unknown but could still be assigned to important marine phyla including prasinophytes, haptophytes, dinoflagellates, stramenopiles, choanoflagellates and acantharians. We also found a novel lineage, closely related to dinoflagellates and not previously described.