SPONTANEOUS PROLIFERATION OF PERIPHERAL-BLOOD LYMPHOCYTES INCREASED IN PATIENTS WITH HTLV-I-ASSOCIATED MYELOPATHY
SPONTANEOUS PROLIFERATION OF PERIPHERAL-BLOOD LYMPHOCYTES INCREASED IN PATIENTS WITH HTLV-I-ASSOCIATED MYELOPATHY
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DOI：
10.1212/wnl.38.8.1302
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发表时间：
1988-08-01
期刊：
影响因子：
9.9
通讯作者：
YAMAMOTO, N
中科院分区：
文献类型：
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作者：
ITOYAMA, Y;MINATO, S;YAMAMOTO, N
我们发现，与HTLV- i血清阳性的非HAM携带者（N = 8）或HTLV- i血清阴性对照（N = 16）相比，14例人t淋巴细胞(HTLV)- i相关（HAM）患者的非刺激（自发）外周血淋巴细胞（PBL）增殖显著增加。HAM患者对植物血凝素、豆豆蛋白A或美洲商陆有丝分裂原的增殖反应降低。在来自HAM患者的未受刺激的PBL培养中，细胞团簇很常见，但在对照组或携带者中却不常见。当贴壁细胞群从培养物中消失时，这种自发的PBL增殖受到抑制。在HAM患者培养3天的细胞上清中，IL-2活性增加，但在对照组培养的细胞中没有。由于IL-2受体阳性细胞在HAM中增加，这种自发的PBL增殖可能是通过IL-2受体的表达对IL-2的反应。
We found unstimulated (spontaneous) peripheral blood lymphocyte (PBL) proliferation significantly increased in 14 patients with human T-lymphotropic virus (HTLV)-I-associated (HAM) compared with findings in HTLV-I-seropositive non-HAM carriers (N = 8) or HTLV-I seronegative controls (N = 16). The proliferative response to phytohemagglutinin, concanavalin A, or pokeweed mitogen was decreased in the HAM patients. Cell clusters were frequent in cultures of unstimulated PBL from the HAM patients, but much less common in the controls or carriers. This spontaneous PBL proliferation was depressed when adherent-cell populations were depleted from the cultures. IL-2 activity increased in the supernatant of 3-day cultured cells from HAM patients, but not in cultured cells from the controls. Since IL-2 receptor positive cells increased in HAM, this spontaneous PBL proliferation is probably a response to IL-2 through the expression of IL-2 receptors.