Green fluorescent protein as a visual marker in somatic hybridization.
Green fluorescent protein as a visual marker in somatic hybridization.
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DOI：
10.1093/aob/mcf054
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发表时间：
2002-04
期刊：
影响因子：
4.2
通讯作者：
O. Olivares-Fuster;Leandro Peña;Nuria Duran-Vila;Luis Navarro
中科院分区：
文献类型：
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作者：
O. Olivares-Fuster;Leandro Peña;Nuria Duran-Vila;Luis Navarro
以转绿色荧光蛋白（GFP）基因的柑橘为亲本进行体细胞融合实验，研究了GFP作为活体标记在原生质体融合、再生和杂种选择中的适用性。在转基因柑橘原生质体、杂种愈伤组织、胚和植株中检测到高水平的GFP表达。研究表明，GFP可用于融合过程的连续监测、杂种菌落和愈伤组织的定位以及体细胞杂种胚和植株的筛选。
Using a transgenic citrus plant expressing Green Fluorescent Protein (GFP) as a parent in somatic fusion experiments, we investigated the suitability of GFP as an in vivo marker to follow the processes of protoplast fusion, regeneration and selection of hybrid plants. A high level of GFP expression was detected in transgenic citrus protoplasts, hybrid callus, embryos and plants. It is demonstrated that GFP can be used for the continuous monitoring of the fusion process, localization of hybrid colonies and callus, and selection of somatic hybrid embryos and plants.