Reversible Photoreaction of a Retinal Photoisomerase, Retinal G Protein-Coupled Receptor RGR
Reversible Photoreaction of a Retinal Photoisomerase, Retinal G Protein-Coupled Receptor RGR
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视网膜光异构酶、视网膜 G 蛋白偶联受体 RGR 的可逆光反应
DOI：
10.1021/acs.biochem.3c00084
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发表时间：
2023
期刊：
影响因子：
2.9
通讯作者：
Keiichi Inoue
中科院分区：
文献类型：
--
作者：
Naoya Morimoto;Takashi Nagata;Keiichi Inoue
视网膜G蛋白偶联受体（RGR）作为视网膜光异构酶在脊椎动物的视觉系统中起着重要作用，它能将全反式视网膜异构化为11-顺式视网膜，维持视紫红质的光敏性。尽管以前的表征牛RGR，鲜为人知的是从其他物种的RGR的光谱特性。此外，11-顺式-视黄醇结合形式的光反应性仍不清楚。在这项研究中，我们发现，人类和鸡RGRs形成蓝色吸收色素类似牛RGR。此外，光谱和生化分析表明，牛和鸡RGRs是一种具有可逆光反应的视紫红质。这些发现提供了深入了解RGR作为视网膜光异构酶的行为，并有助于理解其在视觉系统中的作用。
Retinal G-protein-coupled receptor (RGR) plays a crucial role in the visual system of vertebrates as a retinal photoisomerase, which isomerizes all-trans-retinal to 11-cis-retinal to maintain the photosensitivity of visual rhodopsins. Despite the previous characterization of bovine RGR, little is known about the spectral properties of RGR from other species. In addition, photoreactivity of the 11-cis-retinal-binding form remains unclear. In this study, we revealed that human and chicken RGRs form blue-absorbing pigments similar to bovine RGR. Furthermore, the spectroscopic and biochemical analyses revealed that bovine and chicken RGRs are bistable rhodopsins displaying a reversible photoreaction. These findings provide insight into the behavior of RGR as a retinal photoisomerase and aid in understanding its role in the visual system.