Quantitative analysis of lymphangiogenic markers for predicting metastasis of human gastric carcinoma to lymph nodes
Quantitative analysis of lymphangiogenic markers for predicting metastasis of human gastric carcinoma to lymph nodes
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DOI：
10.1002/ijc.20859
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发表时间：
2005-06-20
影响因子：
6.4
通讯作者：
Chayama, K
中科院分区：
文献类型：
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作者：
Kitadai, Y;Kodama, M;Chayama, K
肿瘤细胞向区域淋巴结的扩散是胃癌转移的早期事件。在我们的研究中，我们评估了淋巴管生成因子和淋巴管内皮标记物在胃癌组织中的表达，并将表达水平与淋巴结转移状态进行比较。我们还研究了原发肿瘤淋巴管密度（LVD）与淋巴结转移的相关性。对39例胃癌患者的胃组织（肿瘤和相应的正常粘膜）进行配对活检。实时定量PCR检测VEGF-C、VEGF-D、VEGFR-3和podoplanin mRNA的表达。VEGF-C在有淋巴结转移的乳腺癌中的表达明显高于无淋巴结转移的乳腺癌。淋巴管内皮标志物VEGFR-3和podoplanin的表达在淋巴结阳性组中也显著更高。用免疫组化方法检测足平面蛋白，发现LVD与淋巴结转移相关。这些结果表明，定量分析胃活检标本中的淋巴管生成标记物可能有助于预测胃癌区域淋巴结转移。(c)2005 Wiley-Liss，Inc.
The spread of tumor cells to regional lymph nodes is an early event of gastric cancer metastasis. In our study, we assessed the expression of lymphangiogenic factors and lymphatic endothelial markers in gastric carcinoma tissues and compared expression levels with the status of lymph node metastasis. We also examined the correlation between lymphatic vessel density (LVD) in primary tumors and lymph node metastasis. Paired biopsy samples (tumor and corresponding normal mucosa) of gastric tissue were obtained from 39 patients with gastric carcinoma. The expression of VEGF-C, VEGF-D, VEGFR-3 and podoplanin mRNAs was assessed by real-time quantitative PCR. The expression of VEGF- C (but not of VEGF-D) was significantly greater in patients with lymph node metastasis than in those without metastasis. The expression of lymphatic endothelial markers VEGFR-3 and podoplanin was also significantly greater in the node-positive group. LVD, as assessed by immunohistochemistry for podoplanin, was correlated with lymph node metastasis. These results indicate that quantitative analysis of lymphangiogenic markers in gastric biopsy specimens may be useful in predicting metastasis of gastric cancer to regional lymph nodes. (c) 2005 Wiley-Liss, Inc.