The Hippo pathway effector Yki downregulates Wg signaling to promote retinal differentiation in the Drosophila eye
The Hippo pathway effector Yki downregulates Wg signaling to promote retinal differentiation in the Drosophila eye
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DOI：
10.1242/dev.117358
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发表时间：
2015-06-01
期刊：
影响因子：
4.6
通讯作者：
Singh, Amit
中科院分区：
文献类型：
--
作者：
Wittkorn, Erika;Sarkar, Ankita;Singh, Amit
进化上保守的河马信号通路被认为在发育过程中调节细胞增殖和维持组织动态平衡。我们发现，河马信号通路的效应者约克(Yki)的激活对果蝇眼睛的生长和分化造成了不同的影响。我们提出的证据支持yki通过下调核心视网膜决定基因来抑制眼睛命运的作用。河马通路的其他上游调节因子介导了yki对视网膜分化的这种影响。在这里，我们表明，在发育中的眼睛，yki可以通过阻止形态发生沟(Mf)进展和R8规范来阻止视网膜分化。MF进展的抑制是由于异位诱导的无翼(Wg)信号和同胸(HTH)，这是眼睛发育的负面调节因素。调节Wg信号可以改变yki介导的眼球命运抑制。此外，在眼睛中由于yki激活而引起的异位HTH诱导依赖于Wg。最后，使用触角命运的标志物Cut(Ct)，我们证明了yki的过度激活抑制了眼睛的命运并没有改变细胞的命运(从眼睛到天线特有的命运)。综上所述，我们提供了yki在细胞命运指定和分化中发挥作用的遗传机制--yki功能的一个新方面，它是从多种模式生物中出现的。
The evolutionarily conserved Hippo signaling pathway is known to regulate cell proliferation and maintain tissue homeostasis during development. We found that activation of Yorkie (Yki), the effector of the Hippo signaling pathway, causes separable effects on growth and differentiation of the Drosophila eye. We present evidence supporting a role for Yki in suppressing eye fate by downregulation of the core retinal determination genes. Other upstream regulators of the Hippo pathway mediate this effect of Yki on retinal differentiation. Here, we show that, in the developing eye, Yki can prevent retinal differentiation by blocking morphogenetic furrow (MF) progression and R8 specification. The inhibition of MF progression is due to ectopic induction of Wingless (Wg) signaling and Homothorax (Hth), the negative regulators of eye development. Modulating Wg signaling can modify Yki-mediated suppression of eye fate. Furthermore, ectopic Hth induction due to Yki activation in the eye is dependent on Wg. Last, using Cut (Ct), a marker for the antennal fate, we show that suppression of eye fate by hyperactivation of yki does not change the cell fate (from eye to antenna-specific fate). In summary, we provide the genetic mechanism by which yki plays a role in cell fate specification and differentiation - a novel aspect of Yki function that is emerging from multiple model organisms.