Analysis of iridoids, secoiridoids and xanthones in Centaurium erythraea, Frasera caroliniensis and Gentiana lutea using LC-MS and RP-HPLC
Analysis of iridoids, secoiridoids and xanthones in Centaurium erythraea, Frasera caroliniensis and Gentiana lutea using LC-MS and RP-HPLC
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DOI：
10.1016/j.jpba.2010.09.030
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发表时间：
2011-02-20
影响因子：
3.4
通讯作者：
Stuppner, Hermann
中科院分区：
文献类型：
--
作者：
Aberham, Anita;Pieri, Valerio;Stuppner, Hermann
本研究建立了一种同时测定百里香、红花龙胆和黄龙胆中活性成分的高效液相色谱方法。在RP-18色谱柱上，以0.085%的邻磷酸水溶液-乙腈为流动相，分离了马钱子酸、4个环烯醚烷类化合物和29个黄原酮。从红景天和红景天的根中分离得到25个口山酮和3个环烯醚萜类化合物，其结构经核磁共振、MS、UV等波谱手段确证。从红曲霉中分离得到1，3，8-三羟基-5，6-二甲氧基口山酮，为一新的口山酮。通过将样品置于光、湿度和不同的温度下来测试分析物的稳定性。贮藏6个月后，观察龙胆苦苷和苦瓜苷的分解情况。在室温和冷藏条件下，苦马豆素的含量几乎没有变化，但高温条件下其含量降低到85%。相反，在长期、冷藏和加速条件下，口山酮是稳定的。所建立的色谱方法已成功地用于三种植物中活性物质的定量。讨论了龙胆科三个成员中多氧杂环己酮的存在和分布，以及它们作为分析标记的意义。(C)2010爱思唯尔B.V.保留所有权利。
This study presents a new and validated HPLC method for the simultaneous determination of bioactive compounds in Centaurium erythraea, Frasera caroliniensis and Gentiana lutea. The iridoid loganic acid, four secoiridoids and 29 xanthones were separated on a RP-18 column, using aqueous o-phosphoric acid (0.085%, v/v) and acetonitrile as mobile phase. Phytochemical investigation of C. erythraea herb and F. caroliniensis roots resulted into isolation of 25 xanthones and three secoiridoids the structure of which was elucidated by spectroscopic means (NMR, MS and UV). 1,3,8-Trihydroxy-5,6-dimethoxyxanthone, isolated from C. erythraea, turned out to be a novel xanthone. The stability of the analytes was tested by subjecting samples to light, moisture and different temperatures. After six months of storage, decomposition of gentiopicroside and sweroside was observed. The swertiamarin content was nearly unchanged when stored at room temperature or in the refrigerator, but high temperature conditions reduced the content to 85%. In contrast, xanthones were stable under long-term, refrigerated and accelerated conditions. The established chromatographic method has been successfully applied for the quantification of the bioactive compounds in the three plants. The presence and distribution of polyoxygenated xanthones within the three members of the Gentianaceae family and their significance as analytical markers are discussed. (C) 2010 Elsevier B.V. All rights reserved.