Effects of O2 and CH4 on presence and activity of the indigenous methanotrophic community in rice field soil
Effects of O2 and CH4 on presence and activity of the indigenous methanotrophic community in rice field soil
复制标题
O2 和 CH4 对稻田土壤中本地甲烷营养群落的存在和活动的影响
DOI：
10.1046/j.1462-2920.2000.00149.x
复制
发表时间：
2000-12-01
影响因子：
5.1
通讯作者：
Conrad, R
中科院分区：
文献类型：
--
作者：
Henckel, T;Roslev, P;Conrad, R
甲烷氧化菌在土壤中的活性和分布取决于CH 4和O-2的有效性。因此，我们研究了高和低CH 4和O-2浓度的4个因子组合下稻田土壤甲烷氧化菌群落的活性和结构。通过变性梯度凝胶电泳（DGGE）解析甲烷氧化菌群体结构，使用靶向16 S rRNA基因的不同PCR引物组，以及编码甲烷氧化菌中关键酶的两个功能基因，即颗粒甲烷单加氧酶（pmoA）和甲醇脱氢酶（mxaF），通过分析磷脂酯连接脂肪酸（PLFA）生物标志物来确定水稻土壤中I型和II型甲烷氧化细菌生物量的变化。I型和II型甲烷氧化菌的甲烷氧化活性测定的相对贡献，通过标记的土壤样品与（CH 4）-C-14，然后通过分析[C-14]-PLFA。高O_2 ~-（20.5%）抑制CH_4氧化，低O_2 ~-（1%）促进CH_4氧化。PCR-DGGE结果表明，不同甲烷氧化菌群落的多样性随CH 4和O-2混合比例的不同而不同，在CH 4浓度较低时比在高浓度时具有更高的多样性，但未检测到I型和II型甲烷氧化菌群落的普遍存在。揭示了CH 4氧化活性在低CH 4混合比（1000 p. p. m. v.）而在CH 4消耗开始期间，无论O-2或CH 4混合比如何。在高甲烷混合比（10 000 p. p. m. v.）下，I型和II型甲烷氧化菌对测量的CH 4代谢的贡献相等。总的来说，I型甲烷氧化菌反应迅速，人口结构的明显变化，并主导了所有四种气体混合物下的活动。II型甲烷氧化菌，另一方面，虽然显然更丰富，总是存在，并表现出很大程度上稳定的人口结构，成为活跃的后，并有助于甲烷氧化活性主要是在高甲烷混合比。
The activity and distribution of methanotrophs in soil depend on the availability of CH4 and O-2. Therefore, we investigated the activity and structure of the methanotrophic community in rice field soil under four factorial combinations of high and low CH4 and O-2 concentrations. The methanotrophic population structure was resolved by denaturant gradient gel electrophoresis (DGGE) with different PCR primer sets targeting the 16S rRNA gene, and two functional genes coding for key enzymes in methanotrophs, i.e. the particulate methane monooxygenase (pmoA) and the methanol dehydrogenase (mxaF), Changes in the biomass of type I and II methanotrophic bacteria in the rice soil were determined by analysis of phospholipid-ester-linked fatty acid (PLFA) biomarkers. The relative contribution of type I and II methanotrophs to the measured methane oxidation activity was determined by labelling of soil samples with (CH4)-C-14 followed by analysis of [C-14]-PLFAs. CH4 oxidation was repressed by high O-2 (20.5%), and enhanced by low O-2 (1%). Depending on the CH4 and O-2 mixing ratios, different methanotrophic communities developed with a higher diversity at low than at high CH4 concentration as revealed by PCR-DGGE, However, a prevalence of type I or II populations was not detected, The [C-14]-PLFA fingerprints, on the other hand, revealed that CH4 oxidation activity was dominated by type I methanotrophs in incubations with low CH4 mixing ratios (1000 p.p.m.v.) and during initiation of CH4 consumption regardless of O-2 or CH4 mixing ratio. At high methane mixing ratios (10 000 p.p.m.v.), type I and II methanotrophs contributed equally to the measured CH4 metabolism. Collectively, type I methanotrophs responded fast and with pronounced shifts in population structure and dominated the activity under all four gas mixtures. Type II methanotrophs, on the other hand, although apparently more abundant, always present and showing a largely stable population structure, became active later and contributed to CH4 oxidation activity mainly under high CH4 mixing ratios.