A microfluidic device to study cancer metastasis under chronic and intermittent hypoxia
A microfluidic device to study cancer metastasis under chronic and intermittent hypoxia
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DOI：
10.1063/1.4898788
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发表时间：
2014-09-01
期刊：
影响因子：
3.2
通讯作者：
Gamcsik, Michael P.
中科院分区：
文献类型：
--
作者：
Acosta, Miguel A.;Jiang, Xiao;Gamcsik, Michael P.
转移性癌细胞必须穿过一个微环境，从肿瘤内的极缺氧到宿主血管内的高氧。肿瘤缺氧可进一步表现为慢性和间歇性缺氧。我们提出了一种微流体装置的设计和表征，该装置可以同时模拟肿瘤内观察到的氧合条件，并模拟细胞迁移和内渗过程。该装置可在癌细胞长期培养过程中产生慢性缺氧的空间氧梯度，产生动态变化的缺氧微环境。(C) 2014 AIP出版有限责任公司
Metastatic cancer cells must traverse a microenvironment ranging from extremely hypoxic, within the tumor, to highly oxygenated, within the host's vasculature. Tumor hypoxia can be further characterized by regions of both chronic and intermittent hypoxia. We present the design and characterization of a microfluidic device that can simultaneously mimic the oxygenation conditions observed within the tumor and model the cell migration and intravasation processes. This device can generate spatial oxygen gradients of chronic hypoxia and produce dynamically changing hypoxic microenvironments in long-term culture of cancer cells. (C) 2014 AIP Publishing LLC.