Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors
Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors
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DOI：
10.1111/gcb.13249
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发表时间：
2016-06-01
影响因子：
11.6
通讯作者：
Thiyagarajan, Vengatesen
中科院分区：
文献类型：
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作者：
Dineshram, Ramadoss;Chandramouli, Kondethimmanahalli;Thiyagarajan, Vengatesen
太平洋牡蛎(Crassostrea Gigas)浮游幼体的变态通过开启固着生命所需的分子机制并建造方解石外壳，为它们复杂的生活史策略奠定了基础。变态成为一个生存瓶颈，它将受到不同人类活动引起的气候变化相关变量的压力。因此，了解变态幼虫如何与新出现的气候变化应激源相互作用是很重要的。为了预测幼虫在未来的海洋中可能受到的影响，我们研究了变态幼虫在多种应激源下蛋白质组的变化：pH降低(pH 7.4)、温度升高(30摄氏度)和盐度降低(15PSU)。使用iTRAQ-LC-MS/MS的定量蛋白质表达谱鉴定了1300多种蛋白质。PH降低通过下调能量产生、代谢和蛋白质合成中的几种蛋白质，从而对变态产生负面影响。然而，在pH值为7.4时，变暖开启了这些下调的通路。在多种应激源作用下，细胞信号、能量产生、生长和发育途径被上调，尽管变态作用仍然减少。尽管缺乏致死效应，但在蛋白质组水平上观察到了对个体和相互作用的气候变化相关应激源的显著生理反应。黄海种群的变态幼体在蛋白质组水平上似乎具有足够的表型可塑性，可以在未来的沿海海洋中生存，但需要付出发育和生理上的代价。
The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 degrees C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ-LC-MS/MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down-regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down-regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up-regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs.