Comparative analyses of bioactive Mammea coumarins from seven parts of Mammea americana by HPLC-PDA with LC-MS.
Comparative analyses of bioactive Mammea coumarins from seven parts of Mammea americana by HPLC-PDA with LC-MS.
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DOI：
10.1021/jf0532462
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发表时间：
2006-05
影响因子：
6.1
通讯作者：
Hui Yang;B. Jiang;K. Reynertson;Margaret J. Basile;E. Kennelly
中科院分区：
文献类型：
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作者：
Hui Yang;B. Jiang;K. Reynertson;Margaret J. Basile;E. Kennelly
美洲野木瓜是一种热带植物，果实可食。Mammea香豆素是2-羟基-Z-肉桂酸内酯的异戊二烯化衍生物，具有生物活性，在三个Clusiaceae属中分布有限。采用定性和定量分析的方法，研究了山榄香豆素类化合物在山榄种核、种皮、果肉、果皮、叶、茎和根中的分布。使用高效液相色谱-光电二极管阵列检测器（HPLC-PDA）和液相色谱-质谱（LC-MS）对美洲大蠊进行定性和定量分析。本文报道了10种主要的mammea香豆素化合物的分离和鉴定，它们是mammea E/BD（1），mammea E/BC（2），mammea E/BA（3），mammea E/BB（4），mammea B/BA hydroxycyclo F（5），mammea B/BD（6），mammea B/BC（7），mammea B/BA（8），mammea B/BB（9）和mammea B/BA cyclo F（10）。美国，并作为标准。对HPLC-PDA方法的灵敏度、线性、回收率、准确度和精密度进行了验证。10种主要香豆素类化合物的总含量（w/w %）分别为1.1%和1.1%。美洲种根中的含量最高（0.75%），其次为叶（0.64%）、种核（0.48%）、果皮（0.11%）、茎（0.08%）、种皮（0.02%）和果肉（<0.01%）。叶和种子核是丰富的和可持续的天然来源mammea香豆素。此外，所描述的HPLC-PDA和LC-MS方法灵敏度高，准确度高，可用于其他样品中的香豆素类化合物的分析。
Mammea americana L. is tropical plant in the Clusiaceae family that bears edible fruit. Mammea coumarins are isoprenylated derivatives of the lactones of the 2-hydroxy-Z-cinnamic acids that are bioactive and have limited distribution in three Clusiaceae genera. Qualitative and quantitative analyses were performed to determine the distribution of mammea coumarins in the seed nucleus, seed coat, fruit flesh, fruit skin, leaf, stem, and root of M. americana using high-performance liquid chromatography-photodiode array detector (HPLC-PDA) and liquid chromatography-mass spectrometry (LC-MS). Ten major mammea coumarins, mammea E/BD (1), mammea E/BC (2), mammea E/BA (3), mammea E/BB (4), mammea B/BA hydroxycyclo F (5), mammea B/BD (6), mammea B/BC (7), mammea B/BA (8), mammea B/BB (9), and mammea B/BA cyclo F (10), were isolated and identified from the seed nucleus of M. americana and employed as standards. The HPLC-PDA method was validated with respect to sensitivity, linearity, recovery, accuracy, and precision. The total content (w/w %) of the 10 major mammea coumarins in M. americana was determined to be highest in the root (0.75%), followed by the leaf (0.64%), seed nucleus (0.48%), fruit skin (0.11%), stem (0.08%), seed coat (0.02%), and fruit flesh (<0.01%). The leaf and seed nucleus are rich and sustainable natural sources of mammea coumarins. Additionally, the described HPLC-PDA and LC-MS methods are sensitive and accurate and can be applied to the analysis of mammea coumarins in other samples.