Anti-biofilm formation of a novel stainless steel against Staphylococcus aureus
Anti-biofilm formation of a novel stainless steel against Staphylococcus aureus
复制标题
新型不锈钢对金黄色葡萄球菌的抗生物膜形成
DOI：
10.1016/j.msec.2015.03.012
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发表时间：
2015-06-01
影响因子：
7.9
通讯作者：
Ren, Guogang
中科院分区：
文献类型：
--
作者：
Nan, Li;Yang, Ke;Ren, Guogang
金黄色葡萄球菌(S.金黄色葡萄球菌）是一种经常发现在金属表面（例如用于保健和食品加工设施的不锈钢）上增殖的细菌。研究表明，一种新型的含铜304型不锈钢（304 CuSS）具有优良的抗菌性能（即对S.金黄色）。本文研究了304 CuSS对S.金黄色葡萄球菌生物被膜形成持续7天。结果表明，与304 SS相比，304 CuSS对固着细菌的抑菌率可达99.9%以上。随着接触时间的延长，304 SS表面生物膜的厚度和尺寸明显增大，生物膜与钢表面的表面自由能变化表明，生物膜的存在增加了生物污染的风险。结果表明，304 CuSS对生物膜的生长和黏附具有较强的抑制作用。304 CuSS与固着细菌细胞接触后的表面自由能在相同培养时间内远低于304 SS。Cu 2+离子的连续溶出很好地证明了富Cu沉淀物在硫暴露后的溶解能力。金黄色葡萄球菌溶液，从3.1 ppm（2天）至4.5 ppm（7天）。对于这种情况的发生，对于304 CuSS可以建立一种假设机制，其中Cu 2+离子是从与微生物的胞外聚合物（EPS）结合的富Cu相中释放的。同时抑制了细胞蛋白/酶的活性，有效地阻止了浮游细菌细胞在304 CuSS金属表面的附着。(C)2015年，作者。由爱思唯尔公司出版
Staphylococcus aureus (S. aureus) is a bacterium frequently found proliferating on metal surfaces such as stainless steels used in healthcare and food processing facilities. Past research has shown that a novel Cu-bearing 304 type stainless steel (304CuSS) exhibits excellent antibacterial ability (i.e. against S. aureus) in a short time period (24 h.). This work was dedicated to investigate the 304CuSS's inhibition ability towards the S. aureus biofilm formation for an extended period of 7 days after incubation. It was found that the antibacterial rate of the 304CuSS against sessile bacterial cells reached over 99.9% in comparison with the 304SS. The thickness and sizes of the biofilms on the 304SS surfaces increased markedly with period of contact, and thus expected higher risk of bio-contamination, indicated by the changes of surface free energy between biofilm and the steel surfaces. The results demonstrated that the 304CuSS exhibited strong inhibition on the growth and adherence of the biofilms. The surface free energy of the 304CuSS after contact with sessile bacterial cells was much lower than that of the 304SS towards the same culture times. The continuously dissolved Cu2+ ions well demonstrated the dissolution ability of Cu-rich precipitates after exposure to S. aureus solution, from 3.1 ppm (2 days) to 4.5 ppm (7 days). For this to occur, a hypothesis mechanism might be established for 304CuSS in which the Cu2+ ions were released from Cu-rich phases that bond with extracellular polymeric substances (EPS) of the microorganisms. And these inhibited the activities of cell protein/enzymes and effectively prevented planktonic bacterial cells attaching to the 304CuSS metal surface. (C) 2015 The Authors. Published by Elsevier B.V.