MODIFICATIONS OF CELL-SURFACE SIALIC ACIDS MODULATE CELL-ADHESION MEDIATED BY SIALOADHESIN AND CD22
MODIFICATIONS OF CELL-SURFACE SIALIC ACIDS MODULATE CELL-ADHESION MEDIATED BY SIALOADHESIN AND CD22
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DOI：
10.1007/bf00731309
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发表时间：
1994-12-01
影响因子：
3
通讯作者：
CROCKER, PR
中科院分区：
文献类型：
--
作者：
KELM, S;SCHAUER, R;CROCKER, PR
已经发现越来越多的哺乳动物细胞粘附分子，包括唾液酸粘附、CD 22和选择素家族，结合含有唾液酸的细胞表面糖缀合物。在这里，我们描述了这种糖的结构多样性如何影响相关分子sialoadhesin和CD 22介导的细胞粘附在小鼠巨噬细胞和B细胞分别。我们表明Neu 5，9Ac（2）的9-0-乙酰基和NeuSGc的N-乙醇酰残基干扰唾液酸粘附素结合。相比之下，与Neu 5Ac相比，CD 22与NeuSGc的结合更强。两个合成的唾液酸测试，只有CD 22绑定的N-甲酰基衍生物，而N-三氟乙酰基残基被唾液酸粘附素接受。唾液酸依赖的细胞粘附现象的调节的潜在意义进行了讨论。
An increasing number of mammalian cell adhesion molecules, including sialoadhesion, CD22 and the family of selectins, have been found to bind cell surface glycoconjugates containing sialic acids. Here we describe how the structural diversity of this sugar influences cell adhesion mediated by the related molecules sialoadhesin and CD22 in murine macrophages and B-cells respectively. We show that the 9-0-acetyl group of Neu5,9Ac(2) and the N-glycoloyl residue of NeuSGc interfere with sialoadhesin binding. In contrast, CD22 binds more strongly to NeuSGc compared to Neu5Ac. Of two synthetic sialic acids tested, only CD22 bound the N-formyl derivative, whereas a N-trifluoroacetyl residue was accepted by sialoadhesin. The potential significance for the regulation of sialic acid dependent cell adhesion phenomena is discussed.