Germ cell proliferation and apoptosis in the developing human ovary
Germ cell proliferation and apoptosis in the developing human ovary
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DOI：
10.1210/jc.2005-0219
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发表时间：
2005-08-01
影响因子：
5.8
通讯作者：
Anderson, RA
中科院分区：
文献类型：
--
作者：
Fulton, N;da Silva, SJM;Anderson, RA
背景：人类卵巢发育过程中生殖细胞增殖和丢失的调控机制尚不清楚。这是特别感兴趣的时间导致了原始卵泡的形成，在18周的妊娠forward.Objective：本研究的目的是确定和量化生殖细胞增殖和凋亡和半胱氨酸天冬氨酸蛋白酶在人类胎儿卵巢的表达。设计：本研究是一项实验室调查。设置：本研究是在一个研究所进行。方法：采用磷酸化组蛋白H3和半胱氨酸蛋白酶-3（caspase-3）的免疫组化定位检测细胞增殖和凋亡。半胱氨酸蛋白酶也被检测到免疫blotting.Results：生殖细胞在有丝分裂的整体比例保持不变，14和19周之间，但表现出越来越多的集群。免疫印迹法检测Caspase-2、-3、-7、-8和-9。生殖细胞凋亡显著增加。妊娠20周的标本显示类似的磷酸化组蛋白H3，但显着降低裂解caspase-3的表达比早期妊娠。切割型caspase-3不表达在卵母细胞已形成的原始follicles.Conclusions：这些结果表明，作为原始卵泡形成的启动和进展，有丝分裂活性和凋亡的那些生殖细胞没有达到明显的保护环境的原始卵泡的增加。
Context: The regulation of germ cell proliferation and loss during human ovarian development is poorly understood. This is of particular interest at the time leading up to the formation of primordial follicles, at 18 wk gestation onward.Objective: The objective of the study was to identify and quantify germ cell proliferation and apoptosis and expression of caspases in the human fetal ovary.Design: This study was a laboratory investigation.Setting: The study was conducted at a research institute.Methods: Cell proliferation and apoptosis were detected using immunohistochemical localization of phosphorylated histone H3 and cleaved caspase-3, respectively. Caspases were also detected by immunoblotting.Results: The overall proportion of germ cells in mitosis remained constant between 14 and 19 wk but showed increasing clustering. Caspase-2, -3, -7, -8, and -9 were detected by immunoblotting. There was a significant increase in germ cell apoptosis. A specimen of 20 wk gestation showed similar phosphorylated histone H3 but markedly lower cleaved caspase-3 expression than earlier gestations. Cleaved caspase-3 was not expressed in oocytes that had formed primordial follicles.Conclusions: These results indicate that as primordial follicle formation is initiated and progresses, there is an increase in both mitotic activity and apoptosis of those germ cells that have not reached the apparently protective environment of the primordial follicle.