Transcriptome Sequencing Reveals Potential Mechanisms of the Maternal Effect on Egg Diapause Induction of Locusta migratoria
Transcriptome Sequencing Reveals Potential Mechanisms of the Maternal Effect on Egg Diapause Induction of Locusta migratoria
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DOI：
10.3390/ijms20081974
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发表时间：
2019-04-02
影响因子：
5.6
通讯作者：
Zhang, Zehua
中科院分区：
文献类型：
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作者：
Hao, Kun;Jarwar, Aftab Raza;Zhang, Zehua
光周期是影响飞蝗子代滞育诱导的最重要的母体因素之一。此前的研究表明叉头盒蛋白O（FOXO）在调节昆虫滞育中发挥重要作用，但光周期如何刺激母性飞蝗通过FOXO信号通路调节下一代卵滞育仍需解决。本研究获得了长、短光周期下成虫卵巢和脂肪体的转录组。在卵巢和脂肪体中总共137个差异表达基因（DEG）中，71个参与FOXO信号通路的DEG可能与滞育诱导密切相关。选择24个关键DEG，并通过qRT-PCR确认其表达谱与转录组结果一致。然后进行RNA干扰以验证视黄酸诱导蛋白基因(rai1)和foxo的功能。在短光周期下，RNAi母蝗rai1基因显着提高了卵滞育率。然而，在短光周期下，通过敲除母蝗中的foxo基因，卵滞育率显着降低。此外，RNAi rai1 还能促进活性氧 (ROS) 和超氧化物歧化酶 (SOD) 活性。我们鉴定了与FOXO途径相关的候选基因，并验证了rai1和foxo仅在短光周期下的滞育调节功能。未来，研究人员可以在该领域开展工作，探索在长光周期下促进滞育诱导的其他因素和基因。
Photoperiod is one of the most important maternal factors with an impact on the offspring diapause induction of Locusta migratoria. Previous studies have shown that forkhead box protein O (FOXO) plays an important role in regulating insect diapause, but how photoperiod stimulates maternal migratory locusts to regulate the next generation of egg diapause through the FOXO signaling pathway still needs to be addressed. In this study, the transcriptomes of ovaries and fat bodies of adult locusts under a long and short photoperiod were obtained. Among the total of 137 differentially expressed genes (DEGs) in both ovaries and fat bodies, 71 DEGs involved in FOXO signaling pathways might be closely related to diapause induction. 24 key DEGs were selected and their expression profiles were confirmed to be consistent with the transcriptome results using qRT-PCR. RNA interference was then performed to verify the function of retinoic acid induced protein gene (rai1) and foxo. Egg diapause rates were significantly increased by RNAi maternal locusts rai1 gene under short photoperiods. However, the egg diapause rates were significantly decreased by knock down of the foxo gene in the maternal locusts under a short photoperiod. In addition, reactive oxygen species (ROS) and superoxide dismutase (SOD) activities were promoted by RNAi rai1. We identified the candidate genes related to the FOXO pathway, and verified the diapause regulation function of rai1 and foxo under a short photoperiod only. In the future, the researchers can work in the area to explore other factors and genes that can promote diapause induction under a long photoperiod.