Isolation and characterization of a novel Staphylococcus aureus bacteriophage, ϕMR25, and its therapeutic potential
Isolation and characterization of a novel Staphylococcus aureus bacteriophage, ϕMR25, and its therapeutic potential
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DOI：
10.1007/s00705-010-0623-2
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发表时间：
2010-03
影响因子：
2.7
通讯作者：
Hiroshi Hoshiba;Jumpei Uchiyama;Shin-ichiro Kato;Takako Ujihara;Asako Muraoka;M. Daibata;H. Wakiguchi;S. Matsuzaki
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文献类型：
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作者：
Hiroshi Hoshiba;Jumpei Uchiyama;Shin-ichiro Kato;Takako Ujihara;Asako Muraoka;M. Daibata;H. Wakiguchi;S. Matsuzaki
用丝裂霉素C诱导从溶原性金黄色葡萄球菌中分离到一株新噬菌体，命名为MMR 25。将其生物学特征与先前描述为原型治疗性噬菌体的WARMMR 11进行比较分析。在形态学上，MyoMR25与MyoMR11（Myoviridae的形态型B1）相似，但在S上具有比MyoMR11更广的宿主范围。金黄色菌株。MR25也可以在S上相乘。溶金菌属其DNA大小为44，342 bp，预计包括70个开放阅读框，不含与毒素或耐药性相关的基因。溶原模块和大多数推定的病毒粒子蛋白基因与WMMR 11的完全不同。尽管它们具有遗传多样性，但腹腔内注射SAMMR 25拯救了接种致死剂量S的小鼠。金黄色葡萄球菌，如对于ESTIMR11的情况。这些结果表明，CD4MR25可能是另一个候选噬菌体治疗。金黄色葡萄球菌感染
A novel bacteriophage, ϕMR25, was isolated from a lysogenicStaphylococcus aureusstrain by mitomycin C induction. Its biological features were analyzed in comparison with ϕMR11, which was described previously as a prototype therapeutic phage. ϕMR25 is morphologically similar to ϕMR11 (morphotype B1 of familyMyoviridae) but has a broader host range than ϕMR11 onS. aureusstrains. ϕMR25 can also multiply onS. aureuslysogens of ϕMR11. Its DNA is 44,342 bp in size, is predicted to include 70 open reading frames, and does not contain genes related to toxin or drug resistance. The lysogenic module and most of the putative virion protein genes are completely different from those of ϕMR11. In spite of their genetic diversity, intraperitoneal administration of ϕMR25 rescued mice inoculated with a lethal dose ofS. aureus, as was the case for ϕMR11. These results suggest that ϕMR25 could be another candidate phage to treatS. aureusinfection.