Japanese Encephalitis Virus in Mosquitoes and Swine in Yunnan Province, China 2009-2010
Japanese Encephalitis Virus in Mosquitoes and Swine in Yunnan Province, China 2009-2010
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DOI：
10.1089/vbz.2012.1016
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发表时间：
2013-01-01
影响因子：
2.1
通讯作者：
Jin, Ning-Yi
中科院分区：
文献类型：
--
作者：
Liu, Hao;Lu, Hui-Jun;Jin, Ning-Yi
2009-2010年对云南省景洪州中国居民区蚊媒和猪媒中乙脑病毒感染情况进行了调查，以确定携带乙脑病毒的人畜共患媒介的频率。总共收集了2.15万只蚊子，并按物种进行了分类，并收集了108只死产仔猪的脑组织。不同种类乙脑病毒的感染率分别为三带喙库蚊13.2%、中华按蚊2.7%、信天鹅0.7%、死产仔猪18.5%。对云南0901和云南0902两个不同季节、不同地区采集的乙型脑炎病毒样品进行了全基因组测序。与24个JEV全序列和93个JEV编码的E基因全序列比较，蚊虫和仔猪的核苷酸同源性分别为97.2-99.6%和94.5-99.7%，推导的氨基酸同源性分别为97.4-98.1%和96.0-98.2%。对云南0901株和云南0902株的全基因组和E基因序列的系统发育分析表明，这两株毒株与我国6株SA14病毒亲缘关系最近，与澳大利亚的Fu、Vellore P20778和日本的石川株以及之前分离的YN86-B8639株亲缘关系较远。基于全基因组的系统发育关系与E基因的系统发育关系相似，表明E基因的系统发育分析将是JEV基因分型的有用方法，但不能更好地了解JEV分离株生物学特性和亲缘关系的潜在变化。
The residential regions of Yunnan province, canton of Jing Hong, in China were surveyed for Japanese encephalitis virus (JEV) infection in mosquito and swine vectors to determine the frequency of JEV-carrying zoonotic vectors in 2009-2010. A total of 21,500 mosquitoes were collected and divided by species, and brain tissue was collected from 108 stillborn piglets. The infection rates for the different JEV species were 13.2% for Culex tritaeniorhynchus, 2.7% for Anopheles sinensis, 0.7% for Armigeres subalbatus, and 18.5% for stillborn piglets. The complete genomes of two JEV samples that were collected in different seasons and different regions, Yunnan 0901 and Yunnan 0902, were sequenced from a pool of Culex mosquitoes and stillborn piglets that had been collected randomly from several piggeries. Multiple sequence alignment with 24 fully-sequenced genes and 93 complete sequences of the JEV-encoded E gene revealed nucleotide homologies ranging from 97.2-99.6% and 94.5-99.7% in mosquitoes and piglets, respectively, and deduced amino acid homologies ranging from 97.4-98.1% and 96.0-98.2%, respectively. Phylogenetic analyses of the Yunnan 0901 and Yunnan 0902 strains' full-length genomes and E gene sequences indicated that these strains are most closely related to six Chinese SA14-derived viruses, and distantly related to the Australian FU, vellore P20778, and Japanese Ishikawa strains, and the previously isolated YN86-B8639 strains. The phylogenetic relationships based on the full-length genome were similar to those found for the E gene, indicating that phylogenetic analysis of the E gene will be a useful approach for genotyping of JEV, but not to better understand the potential changes in the biological characteristics and genetic relationship of JEV isolates.