Spontaneous Regeneration after Hypoglossal Nerve Injury with Long Nerve Resection in Rats
Spontaneous Regeneration after Hypoglossal Nerve Injury with Long Nerve Resection in Rats
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DOI：
10.1007/s11062-024-09946-6
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发表时间：
2024-04
期刊：
影响因子：
0.5
通讯作者：
N. Fukushima;N. Sumitomo;A. Nagira;Y. Ichinose;A. Kakegawa
中科院分区：
文献类型：
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作者：
N. Fukushima;N. Sumitomo;A. Nagira;Y. Ichinose;A. Kakegawa
神经切除间隙较短的周围神经损伤可发生自发神经再生，而神经切除间隙较长的周围神经损伤不能发生再生。我们研究了通过切除一段较长的神经段来模拟成年大鼠严重神经损伤后的自发神经再生。在切除长度为0、3、6、9或12 mm的舌下（XII）神经后，用1,1 ‘二十八烷基-3,3,3 ’，3 ' -四甲基多碳青酸高氯酸酯（DiI）标记XII核中的运动神经元；后者在神经切除12周后多次注入舌内。在0 mm长度神经切除的大鼠中，dii标记的XII神经元数量与对照组相同。在3 mm神经切除大鼠中，dii标记的神经元数量略少于对照组，且明显少于对照组和6、9 mm神经切除大鼠。此外，在12 mm神经切除的大鼠中，dii标记的神经元数量很少，或仅观察到单个细胞。我们认为，在中重度长神经间隙的XII神经损伤后，XII运动神经元可能发生自发神经再生。
Spontaneous neural regeneration may occur in peripheral nerve injury after nerve resection with a relatively short gap, whereas regeneration does not occur after nerve resection with a relatively long gap. We have examined spontaneous neural regeneration after severe nerve injury modeled by the resection of a relatively long nerve segment in adult rats. After hypoglossal (XII) nerve resection at lengths of 0, 3, 6, 9, or 12 mm, motoneurons in the XII nucleus were labeled with 1,1’dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlolate (DiI); the latter was multiply injected into the tongue 12 weeks after the nerve resection. In the 0 mm length nerve-resected rats that were transected, the numbers of DiI-labeled XII neurons were as high as that in the control. In the 3 mm nerve-resected rats, the number of DiI-labeled neurons was slightly less than that in the control, and the numbers were clearly smaller than that in the control and in the 6 and 9 mm nerve-resected rats. Moreover, in the 12 mm nerve-resected rats, the number of DiI-labeled neurons was minimal, or only single such cells were observed. We conclude that spontaneous neural regeneration of the XII motoneurons might occur after moderately severe XII nerve injuries with a long nerve gap.