MICROSATELLITE INSTABILITY IN JAPANESE GASTRIC-CANCER
MICROSATELLITE INSTABILITY IN JAPANESE GASTRIC-CANCER
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DOI：
10.1002/1097-0142(19950315)75:6
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发表时间：
1995-03-15
期刊：
影响因子：
6.2
通讯作者：
AKIYOSHI, T
中科院分区：
文献类型：
--
作者：
NAKASHIMA, H;INOUE, H;AKIYOSHI, T
背景最近的研究表明，微卫星在各种类型的癌症中是不稳定的，并且微卫星位点的这种遗传不稳定性（微卫星不稳定性）被认为在癌症的发展中起重要作用。然而，微卫星不稳定性在胃癌中的临床病理学意义尚不明确。为了阐明遗传不稳定性在胃癌发生发展中的作用，采用聚合酶链反应（PCR）为基础的方法，使用新鲜冷冻的肿瘤-正常配对样本检测了25例胃癌中微卫星不稳定性的存在。微卫星不稳定性定义为肿瘤在两个或多个微卫星位点上显示改变的带型。胃癌病例中微卫星不稳定的发生率为25例患者中的4例（16%），26例癌症中的4例（15%）。在老年组和淋巴结转移阴性组中，微卫星不稳定性的发生率均显著增高（P < 0.01和P < 0.05）。所有显示微卫星不稳定性的胃癌患者淋巴或静脉渗透均为阴性。因此，观察到微卫星不稳定性与无淋巴渗透的统计学显著相关（P < 0.05）。本研究揭示了胃癌患者中罕见的淋巴结转移和淋巴管浸润，表明微卫星不稳定性。虽然检查病例的数量很少，但这些发现表明，显示微卫星不稳定性的胃癌可能因此表现为恶性程度较低的方式。
Background. Recent studies have shown that microsatellites are unstable in various types of cancers, and such genetic instability at the microsatellite loci (microsatellite instability) has been considered to play an important role in the development of cancer. However, the clinicopathologic significance of microsatellite instability in gastric cancer has not been clarified.Methods. To elucidate the role of genetic instability in the development of gastric cancer, the presence of microsatellite instability was examined in 25 cases of gastric cancer using fresh-frozen tumor-normal paired samples using a polymerase chain reaction (PCR)-based method. Microsatellite instability was defined as tumors that showed altered banding patterns at two or more microsatellite loci.Results. The incidence of microsatellite instability in gastric cancer cases was 4 of 25 patients (16%) and 4 of 26 cancers (15%). A significantly high incidence of microsatellite instability was observed in both the elderly (P < 0.01) and in lymph node metastasis-negative patients (P < 0.05). All patients with gastric cancer showing microsatellite instability were negative for lymphatic or venous permeation. A statistically significant association of microsatellite instability with no lymphatic permeation was thus observed (P < 0.05).Conclusions. This study revealed infrequent lymph node metastasis and lymph vessel invasion in the patients with gastric cancer demonstrating microsatellite instability. Although the number of examined cases was small, these findings suggest that gastric cancer that shows microsatellite instability may thus behave in a less malignant manner.