Phytochemical Fingerprinting to Thwart Black Cohosh Adulteration: a 15 Actaea Species Analysis
Phytochemical Fingerprinting to Thwart Black Cohosh Adulteration: a 15 Actaea Species Analysis
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DOI：
10.1002/pca.1285
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发表时间：
2011-07-01
影响因子：
3.3
通讯作者：
Kennelly, Edward J.
中科院分区：
文献类型：
--
作者：
Jiang, Bei;Ma, Chunhui;Kennelly, Edward J.
介绍-黑升麻产品的流行使用(总状花序Actaea racemosa L.，syn.总状升麻(cimicifuga racemosa L.)随着对雌激素疗法替代品的需求增加，这种情况也在增长。安全使用的关键是保证纯正、高质量的产品。由于报告服用黑升麻的患者出现肝脏毒性，人们对黑升麻的安全性提出了质疑；随后的评估发现，一些产品掺入了其他相关草本物种。正确的植物种类鉴定是生产安全的黑升麻制品的关键第一步。目的-建立黑升麻与其他品种(美洲和亚洲)的分析方法，使黑升麻成为商品黑升麻制品中的掺假物。材料和方法-从北美和亚洲采集了15种升麻，并利用高效液相-PDA和LC-MS技术建立了这些样品的植物化学指纹图谱。结果-多酚和三萜苷的高效液相色谱和LC-MS指纹图谱显示了明显的模式，使黑升麻与大多数其他种类的升麻区别开来。两个标记化合物，cimifugin和cimiracemoside F，被发现是区分黑升麻和大多数亚洲升麻属植物的重要成分。结论：植物化学指纹图谱是一种实用、可靠的黑升麻鉴别方法，可与商品黑升麻制品中日益增多的掺假升麻属植物区分开来。这应有助于继续开发高质量、纯正的黑升麻产品。版权所有(C)2011 John Wiley&Sons，Ltd.
Introduction - The popular use of black cohosh products (Actaea racemosa L., syn. Cimicifuga racemosa L.) is growing as the demand for alternatives to estrogen therapy has increased. Critical to safe use is the assurance of unadulterated, high-quality products. Questions have been raised about the safety of black cohosh due to cases of liver toxicity in patients who reported taking it; subsequent evaluation found some products to be adulterated with other related herbal species. Correct plant species identification is a key first step for good manufacturing practices of safe black cohosh products.Objectives - To develop analytical methods which distinguish black cohosh from other species (American and Asian) of Actaea increasingly found as adulterants in commercially available black cohosh products.Material and methods - Fifteen species of Actaea were collected from North America and Asia, and the phytochemical fingerprints of these samples were established using HPLC-PDA and LC-MS techniques.Results - The HPLC and LC-MS fingerprints for polyphenols and triterpene glycosides revealed distinct patterns that make black cohosh clearly distinguishable from most other species of Actaea. Two marker compounds, cimifugin and cimiracemoside F, were found to be important to distinguish black cohosh from most Asian species of Actaea. Formononetin was not found from either Asian or American species of Actaea.Conclusions - Phytochemical fingerprinting is a practical, reliable method for authenticating black cohosh and distinguishing it from other species of Actaea increasingly found as adulterants in commercially available black cohosh products. This should facilitate the continued development of high-quality, unadulterated black cohosh products. Copyright (C) 2011 John Wiley & Sons, Ltd.