Preparation of injectable 3D-formed β-tricalcium phosphate bead/alginate composite for bone tissue engineering
Preparation of injectable 3D-formed β-tricalcium phosphate bead/alginate composite for bone tissue engineering
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DOI：
10.4012/dmj.27.827
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发表时间：
2008-11-01
影响因子：
2.5
通讯作者：
Satoh, Tazuko
中科院分区：
文献类型：
--
作者：
Matsuno, Tomonori;Hashimoto, Yoshiya;Satoh, Tazuko
以β-磷酸三钙（β-TCP）为固相，海藻酸钠为凝胶相，制备了一种新型的可注射性骨组织工程材料。为了制备瞬时形成的复合支架，将所有氯化钙水溶液在β-TCP珠的表面上干燥并与藻酸钠溶液交联，从而形成稳定的β-TCP珠和藻酸盐凝胶，其可通过注射器注射。这种可生物降解的复合材料是一种三维（3D）材料，可用作骨组织工程的可注射支架。特别地，具有2.0重量%藻酸盐浓度的复合物在干燥条件下表现出69 kPa的压缩强度，这显著高于1.0重量%所表现出的压缩强度。此外，间充质干细胞（MSC）在复合材料中进行3D培养，然后研究成骨标志物。将复合材料植入皮下进行体内实验。结果表明，该支架为成骨分化提供了支持。鉴于所获得的令人鼓舞的结果，这种新型的可注射复合材料可能是有用的骨组织工程。
A novel, injectable bone tissue engineering material was developed that consisted of beta-tricalcium phosphate (beta-TCP) beads as the solid phase and alginate as the gel phase. To prepare the instantaneously formed composite scaffold, all aqueous calcium chloride solution was dried on the surface of beta-TCP beads and crosslinked with an alginic acid sodium solution, thereby forming stable beta-TCP beads and alginate gel which were injectable via a syringe. This biodegradable composite was a three-dimensional (3D) material that could be used as an injectable scaffold for bone tissue engineering. In particular, the composite with 2.0 wt% alginate concentration exhibited a compressive strength of 69 kPa in dry conditions, which was significantly higher than that exhibited by 1.0 wt%. Furthermore, mesenchymal stem cells (MSC) were 3D cultured within the composite and then investigated for osteogenic markers. MSC-loaded composite Was subjected to scanning electron microscope (SEM) examination and implanted subcutaneously for in vivo experiment. Results showed that the scaffold provided support for osteogenic differentiation. In light of the encouraging results obtained, this novel injectable composite material may be useful for bone tissue engineering.