Genome-wide analysis of alternative splicing differences between oocyte and zygote
Genome-wide analysis of alternative splicing differences between oocyte and zygote
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卵母细胞和受精卵之间选择性剪接差异的全基因组分析
DOI：
10.1093/biolre/ioaa004
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发表时间：
2020-05-01
影响因子：
3.6
通讯作者：
Liu, Xu
中科院分区：
文献类型：
--
作者：
Cheng, Rui;Zheng, Xiaoman;Liu, Xu
mRNA前体的选择性剪接（AS）允许从单个初级转录物合成多个mRNA，显著扩展了高等真核生物基因组的信息含量和调控可能性。在哺乳动物的发育过程中，AS在不同的生理过程中驱动某些决定性的变化。随着发育的进展，母合子转换（MZT）将触发两个过程：消除母体mRNA的子集和合子基因组的转录开始。最近的高通量技术进步促进了全基因组AS，而其在小鼠卵母细胞过渡到受精卵阶段的分析还没有报道。我们提出了一个高分辨率的全球分析AS转换，发现广泛的AS小鼠卵母细胞和受精卵之间的转换。逆转录-聚合酶链反应分析进一步证实了AS模式的差异。小鼠卵母细胞中具有特异性AS事件的许多基因在卵母细胞和受精卵之间存在差异表达，但只有少数受精卵中具有特异性AS事件的基因在卵母细胞和受精卵之间存在差异表达。我们提供了一个景观的AS事件在小鼠卵母细胞和受精卵。我们的研究结果推进了对小鼠受精过程中AS转换及其对MZT和进一步发育的潜在功能的理解。
Alternative splicing (AS) of mRNA precursors allows the synthesis of multiple mRNAs from a single primary transcript, significantly expanding the information content and regulatory possibilities of higher eukaryotic genomes. During mammalian development, AS drives certain decisive changes in different physiological processes. As development progresses, the maternal-to-zygotic transition (MZT) will trigger two processes: elimination of a subset of maternal mRNA and transcription of the zygote genome begins. Recent high-throughput technological advancements have facilitated genome-wide AS, whereas its analysis in mouse oocyte transition to the zygote stage has not been reported. We present a high-resolution global analysis of AS transitions and discovered extensive AS transitions between mouse oocyte and zygote. The difference of AS patterns was further confirmed using reverse transcription-polymerase chain reaction analysis. Many genes with specific AS events in mouse oocytes are differentially expressed between oocyte and zygote, but only a few genes with specific AS events in zygote are differentially expressed between oocyte and zygote. We provide a landscape of AS events in mouse oocyte and zygote. Our results advance the understanding of AS transitions during mouse fertilization and its potential functions for MZT and further development.Summary SentenceAS events that are differentially expressed between oocyte and zygote are important in the regulation of embryo development.