EST sequencing and microarray analysis of the floral transcriptome of Eustoma grandiflorum
EST sequencing and microarray analysis of the floral transcriptome of Eustoma grandiflorum
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DOI：
10.1016/j.scienta.2011.12.024
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发表时间：
2012-09-06
影响因子：
4.3
通讯作者：
Miyamoto, Kentaro
中科院分区：
文献类型：
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作者：
Kawabata, Saneyuki;Li, Yuhua;Miyamoto, Kentaro
全基因组DNA序列资源有望增强我们对植物发育的分子基础的理解，并导致园艺作物理想性状的改进。在这项研究中，我们测序了643,366 ESTs从洋桔梗花使用一个标准化的cDNA文库构建几个不同的花发育阶段，一天中的时间，和授粉雄蕊。这些序列被组装成63，401个重叠群和242，212个单例。BlastX搜索GenBank数据库中的所有重叠群，将65%的重叠群与注册序列匹配，而35%没有匹配。GO定位将63，401个重叠群中的48%分配给GO术语。微阵列分析表明，随着花的发育，基因的亚群被上调或下调。上调基因富集了与（1）寡肽转运、（2）对茉莉酸刺激的响应和（3）细胞壁修饰相关的GO术语，而下调基因富集了与（1）次生代谢（如类黄酮生物合成过程和萜类生物合成过程）和（2）表皮细胞修饰相关的GO术语。(C)2011 Elsevier B. V.保留所有权利。
Genome-wide DNA sequence resources are expected to enhance our understanding of the molecular basis of plant development and lead to improvements in desirable traits in horticultural crops. In this study, we sequenced 643,366 ESTs from Eustoma grandiflorum flowers using a normalised cDNA library constructed for several different flower development stages, times of day, and pollinated stamens. The sequences were assembled into 63,401 contigs and 242,212 singletons. BlastX searches for all of the contigs in the GenBank database matched 65% of the contigs to registered sequences, while 35% presented no hits. GO mapping assigned 48% of the 63,401 contigs to GO terms. Microarray analysis showed that subsets of genes were up- or downregulated as the flower developed. The upregulated genes were enriched for GO terms related to (1) oligopeptide transport, (2) response to jasmonic acid stimulus, and (3) cell wall modification, whereas the downregulated genes were enriched for GO terms involved in (1) secondary metabolism, such as the flavonoid biosynthetic process and the terpenoid biosynthetic process, and (2) epidermal cell modification. (C) 2011 Elsevier B.V. All rights reserved.