Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci
Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci
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DOI：
10.1046/j.1365-313x.1996.10040655.x
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发表时间：
1996-10-01
期刊：
影响因子：
7.2
通讯作者：
Koornneef, M
中科院分区：
文献类型：
--
作者：
LeonKloosterziel, KM;Gil, MA;Koornneef, M
分离了内源脱落酸（阿坝）水平降低的新的拟南芥突变体。在赤霉素生物合成抑制剂多效唑的存在下，这些在发芽筛选中被选择。另一个突变体是在NaCl耐受性筛选中分离的。ABA缺乏症是由两个单基因隐性突变引起的，aba2和aba3，都位于1号染色体上。这些突变体表现出已知的ABA缺乏症特征表型：种子休眠减少和水分过多损失，导致枯萎表型。结合不同阿坝突变的双突变分析表明突变的渗漏性质。
Novel Arabidopsis mutants with lowered levels of endogenous abscisic acid (ABA) were isolated. These were selected in a screen for germination in the presence of the gibberellin biosynthesis inhibitor paclobutrazol. Another mutant was isolated in a screen for NaCl tolerance. The ABA-deficiency was caused by two monogenic, recessive mutations, aba2 and aba3, that were both located on chromosome 1. The mutants showed a phenotype that is known to be characteristic for ABA-deficiency: a reduced seed dormancy and excessive water loss, leading to a wilty phenotype. Double mutant analysis, combining different aba mutations, indicated the leaky nature of the mutations.