Horizontal Boundary Cells, a Special Group of Somitic Cells, Play Crucial Roles in the Formation of Dorsoventral Compartments in Teleost Somite
Horizontal Boundary Cells, a Special Group of Somitic Cells, Play Crucial Roles in the Formation of Dorsoventral Compartments in Teleost Somite
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DOI：
10.1016/j.celrep.2019.03.068
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发表时间：
2019-04-16
期刊：
影响因子：
8.8
通讯作者：
Takeda, Hiroyuki
中科院分区：
文献类型：
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作者：
Abe, Kota;Shimada, Atsuko;Takeda, Hiroyuki
建立稳健的基因表达边界对于在发育过程中创建复杂的形态至关重要。然而，边界形成的机制仅在少数模型系统中得到了广泛研究。我们检查了 zic1/zic4 表达边界的建立，该边界划分青鳉鱼 (Oryzias latipes) 整个躯干的背腹边界，并确定了称为水平边界细胞 (HBC) 的皮肌节细胞亚群，作为边界形成的关键参与者。胚胎学和遗传分析表明，HBC 在该过程的两个主要事件（即精炼和维护）中发挥着至关重要的作用。在改进中，HBC 可以通过表达 Hhip 作为化学屏障，抵御来自神经管的 Wnt。在后期阶段，HBC 通过分化为水平肌隔以物理方式抑制跨边界的细胞混合来参与边界的维持。这些发现揭示了硬骨鱼躯干背腹边界的特殊边界细胞机制。
Establishment of robust gene expression boundary is crucial for creating elaborate morphology during development. However, mechanisms underlying boundary formation have been extensively studied only in a few model systems. We examined the establishment of zic1/zic4-expression boundary demarcating dorsoventral boundary of the entire trunk of medaka fish (Oryzias latipes) and identified a subgroup of dermomyotomal cells called horizontal boundary cells (HBCs) as crucial players for the boundary formation. Embryological and genetic analyses demonstrated that HBCs play crucial roles in the two major events of the process, i.e., refinement and maintenance. In the refinement, HBCs could serve as a chemical barrier against Wnts from the neural tube by expressing Hhip. At later stages, HBCs participate in the maintenance of the boundary by differentiating into the horizontal myoseptum physically inhibiting cell mixing across the boundary. These findings reveal the mechanisms underlying the dorsoventral boundary in the teleost trunk by specialized boundary cells.