Extraction and determination of chloramphenicol in feed water, milk, and honey samples using an ionic liquid/sodium citrate aqueous two-phase system coupled with high-performance liquid chromatography
Extraction and determination of chloramphenicol in feed water, milk, and honey samples using an ionic liquid/sodium citrate aqueous two-phase system coupled with high-performance liquid chromatography
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使用离子液体/柠檬酸钠水两相系统与高效液相色谱联用萃取和测定给水、牛奶和蜂蜜样品中的氯霉素
DOI：
10.1007/s00216-010-4376-2
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发表时间：
2011-01-01
影响因子：
4.3
通讯作者：
Xie, Xue-qiao
中科院分区：
文献类型：
--
作者：
Han, Juan;Wang, Yun;Xie, Xue-qiao
以咪唑离子液体(1-丁基-3-甲基咪唑四氟硼酸盐，[Bmim]BF4)和有机盐(Na3C6H5O7)为双水相体系，建立了一种绿色、简便、无毒、灵敏的样品前处理与高效液相色谱联用分析氯霉素(CAP)的方法。研究了盐的种类和用量、pH值、[Bmim]BF4的体积和萃取温度等因素对CAP分配行为的影响。CAP的萃取效率随着温度的升高和[Bmim]BF4体积的增大而增大。热力学研究表明，疏水相互作用是主要驱动力，静电相互作用和盐析效应对CAP的转移也很重要。在最佳条件下，一步萃取法可将90.1%的CAP提取到富离子液体相。该方法用于饲料水、牛奶和蜂蜜中草甘膦的测定，线性范围为2~1,000 ng m L−1，检出限为0.3 ng m L−1，定量限为1.0 ng m L−1。对实际饲料水、牛奶和蜂蜜水样的回收率为90.4%~102.7%。这种新工艺更简单，更环保，在抗生素分离方面有重要的应用。图ILATPS中分离目标分子的示意图
A green, simple, non-toxic, and sensitive sample pretreatment procedure coupled with high-performance liquid chromatography (HPLC) was developed for the analysis of chloramphenicol (CAP) that exploits an aqueous two-phase system based on imidazolium ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate, [Bmim]BF4) and organic salt (Na3C6H5O7) using a liquid–liquid extraction technique. The influence factors on partition behaviors of CAP were studied, including the type and amount of salts, the pH value, the volume of [Bmim]BF4, and the extraction temperature. Extraction efficiency of the CAP was found to increase with increasing temperature and the volume of [Bmim]BF4. Thermodynamic studies indicated that hydrophobic interactions were the main driving force, although electrostatic interactions and salting-out effects were also important for the transfer of the CAP. Under the optimal conditions, 90.1% of the CAP could be extracted into the ionic liquid-rich phase in a single-step extraction. This method was practical when applied to the analysis of CAP in feed water, milk, and honey samples with a linear range of 2~1,000 ng mL−1. The method yielded a limit of detection of 0.3 ng mL−1and a limit of quantification of 1.0 ng mL−1. The recovery of CAP was 90.4–102.7% from aqueous samples of real feed water, milk, and honey samples by the proposed method. This novel process is much simpler and more environmentally friendly and is suggested to have important applications for the separation of antibiotics.FigureThe schematic diagram of separation target molecule in ILATPS