Regulation of repressible acid phosphatase gene transcription in Saccharomyces cerevisiae.
Regulation of repressible acid phosphatase gene transcription in Saccharomyces cerevisiae.
复制标题
酿酒酵母中可抑制酸性磷酸酶基因转录的调节。
DOI：
10.1128/mcb.5.8.2131-2141.1985
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发表时间：
1985
影响因子：
5.3
通讯作者：
Bostian,KA
中科院分区：
文献类型：
--
作者：
Lemire,JM;Willcocks,T;Halvorson,HO;Bostian,KA
我们通过分析先前分离的和基因上明确定义的影响APase表达的调控基因突变，在分子水平上研究了负责调控酿酒酵母中可阻遏酸性磷酸酶(APase；正磷酸单酯磷酸水解酶[ACID BEPTIME，EC 3.1.3.2])转录的遗传系统。这些突变体确定了大量的正性(PHO4，PHO2，PHO81)和负性(PHO80，PHO85)调节位，分布在酵母基因组中。我们发现，这些正负调控基因的相互作用发生在APase基因转录之前或过程中，它们的功能都是正常调节mRNA合成所必需的。生化证据表明，它们编码的调控基因产物是结构性表达的。对APase合成的更详细的研究是一个条件PHO80(Ts)突变体，表明无论是PHO4还是APase mRNA合成所需的任何其他蛋白质因子都不受PHO80的转录调控。此外，在没有PHO80的情况下，被认为是PI的代谢物的辅阻遏子并不抑制它们在APase转录激活中的功能。
We examined the genetic system responsible for transcriptional regulation of repressible acid phosphatase (APase; orthophosphoric-monoester phosphohydrolase [acid optimum, EC 3.1.3.2]) inSaccharomyces cerevisiaeat the molecular level by analysis of previously isolated and genetically well-defined regulatory gene mutants known to affect APase expression. These mutants identify numerous positive- (PHO4,PHO2,PHO81) and negative-acting (PHO80,PHO85) regulatory loci dispersed throughout the yeast genome. We showed that the interplay of these positive and negative regulatory genes occurs before or during APase gene transcription and that their functions are all indispensible for normal regulation of mRNA synthesis. Biochemical evidence suggests that the regulatory gene products they encode are expressed constitutively. More detailed investigation of APase synthesis is a conditionalPHO80(Ts) mutant indicated that neitherPHO4nor any other protein factor necessary for APase mRNA synthesis is transcriptionally regulated byPHO80. Moreover, in the absence ofPHO80, the corepressor, presumed to be a metabolite of Pi, did not inhibit their function in the transcriptional activation of APase.