Architectural analysis, viability assessment and growth kinetics of Candida albicans and Candida glabrata biofilms
Architectural analysis, viability assessment and growth kinetics of Candida albicans and Candida glabrata biofilms
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DOI：
10.1016/j.archoralbio.2009.08.002
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发表时间：
2009-11-01
影响因子：
3
通讯作者：
Samaranayake, L. P.
中科院分区：
文献类型：
--
作者：
Seneviratne, C. J.;Silva, W. J.;Samaranayake, L. P.
人体真菌病原体念珠菌能够在几乎所有的医疗器械中形成生物膜。事实上，生物膜形成是微生物的主要毒力属性，并且是大多数人类感染的原因。因此，了解与生物膜发展有关的过程是设计预防或根除生物膜相关感染的新策略的重要先决条件。在本研究中，我们使用了一系列传统和新型的分析工具，以获得念珠菌生物膜发展的全面看法。菌落形成单位计数、比色（XTT）测定、扫描电子显微镜（SEM）和新型共聚焦激光扫描显微镜（CLSM）结合COMSTAT软件分析用于评价两种念珠菌属（C.白色念珠菌和C.光滑的从初始粘附步骤（1.5 h）至72 h评价聚苯乙烯基底上的生物膜生长动力学。这些分析揭示了实质性的种间和种内差异的时间组织念珠菌生物膜结构，空间性和细胞活力，而达到成熟期内的48小时，在聚苯乙烯基板。尽管总体趋势似乎相同，但不同方法的生长动力学存在显著差异。COMSTAT软件提供的详细结构分析证实了SEM和CSLM的观点。这些分析为真菌生物膜的后续分子研究奠定了基础。(C)2009爱思唯尔有限公司保留所有权利。
The human fungal pathogen Candida is able to form biofilms in almost all the medical devices in cur-rent use. indeed, biofilm formation is a major virulence attribute of microorganisms and account for a majority of human infections. Therefore, understanding processes appertaining to biofilm development is an important prerequisite for devising new strategies to prevent or eradicate biofilm-related infections. In the present study we used an array of both conventional and novel analytical tools to obtain a comprehensive view of Candida biofilm development. Enumeration of colony forming units, colorimetric (XTT) assay, Scanning Electron Microscopy (SEM) and novel Confocal Laser Scanning Microscopy (CLSM) coupled with COMSTAT software analyses were utilised to evaluate growth kinetics; architecture and viability of biofilms of a reference (ATCC) and a clinical strain each of two Candida species, C. albicans and C. glabrata. Biofilm growth kinetics on a polystyrene substrate was evaluated from the initial adhesion step (1.5 h) up to 72 h. These analyses revealed substantial inter- and intra-species differences in temporal Organisation of Candida biofilm architecture, spatiality and cellular viability, while reaching maturity within a period of 48 h, on a polystyrene substrate. There were substantial differences in the growth kinetics upon methodology, although general trend seemed to be the same. Detailed architectural analysis provided by COMSTAT software corroborated the SEM and CSLM views. These analyses may provide a strong foundation for down stream molecular work of fungal biofilms. (C) 2009 Elsevier Ltd. All rights reserved.