A lytic bacterium's potential application in biofuel production through directly lysing the diatom Phaeodactylum tricornutum cell
A lytic bacterium's potential application in biofuel production through directly lysing the diatom Phaeodactylum tricornutum cell
复制标题
裂解细菌通过直接裂解三角褐指藻细胞在生物燃料生产中的潜在应用
DOI：
10.1016/j.algal.2015.08.023
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发表时间：
2015-11-01
影响因子：
5.1
通讯作者：
Zheng, Tianling
中科院分区：
文献类型：
--
作者：
Chen, Zhangran;Zhang, Bangzhou;Zheng, Tianling
分离出能够打破藻类细胞壁的微生物将有助于释放淀粉和脂质分子，用于生物燃料生产。在这项研究中，发现了一种特殊的噬菌斑形成细菌，形成直径约2厘米的噬菌斑，目标是硅藻三角褐指藻。为了监测细菌对三角褐指藻的动态影响，通过荧光显微镜和变性梯度凝胶电泳分析了来自正常藻类培养物和裂解物的样品。DGGE结果表明，正常藻培养物和溶胞产物样品之间没有差异条带，表明该细菌的丰度较低。尝试了几种不同的培养基，并使用空斑形成方法进一步研究了从裂解物中获得的细菌。最后，在含有三角褐指藻细胞的培养基上生长的一个分离的细菌显示出特殊的裂解活性。序列分析和系统发育分析结果表明，该细菌属于杜鹃花科Labrenzia属。这项研究证明了一种裂解细菌的分离，该细菌具有通过三角褐指藻“裂解”生产生物燃料的潜力。(C)2015年爱思唯尔B。V.保留所有权利。
The isolation of microorganisms able to break the cell wall of algal species would contribute to the release of starch and lipid molecules for biofuel production. In this study, a special plaque-forming bacterium, forming plaques of about 2 cm in diameter, was found to target the diatom Phaeodactylum tricornutum. To monitor the dynamic effects of the bacterium on P. tricornutum, samples from both normal algal cultures and lysates were analyzed by fluorescence microscopy and denaturing gradient gel electrophoresis. The DGGE results showed that there was no different band between normal algal cultures and lysate samples, suggesting the bacterium's low abundance. Several different culture mediums were tried, and the obtained bacteria from the lysates were further investigated using the plaque-forming method. Finally, one isolated bacterium grown on culture medium containing P. tricornutum cells showed the special lytic activity. The results from sequence and phylogenetic analysis show that this bacterium belongs to the genus of Labrenzia in the family Rhodobacteraceae. This study demonstrates the isolation of a lytic bacterium that has a potential for biofuel production through P. tricornutum 'cracking'. (C) 2015 Elsevier B. V. All rights reserved.