Luteinizing hormone stimulates ingression of mural granulosa cells within the mouse preovulatory follicle†.
Luteinizing hormone stimulates ingression of mural granulosa cells within the mouse preovulatory follicle†.
复制标题
黄体生成素刺激小鼠排卵前卵泡内壁颗粒细胞的侵入。
DOI：
10.1093/biolre/ioad142
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发表时间：
2024
影响因子：
3.6
通讯作者：
Jaffe,LaurindaA
中科院分区：
文献类型：
--
作者：
Owen,CorieM;Jaffe,LaurindaA
黄体生成素（LH）通过作用于哺乳动物卵泡中包围卵母细胞的壁粒细胞中的受体而诱导排卵。然而，关于黄体生成素受体的激活如何改变卵泡的结构，使卵母细胞被释放，卵泡残留物转化为黄体，仍有许多未知之处。本研究表明，排卵前LH的激增刺激表达LH受体的颗粒细胞，最初几乎完全位于壁粒的外层，迅速向内延伸，插入其他细胞之间。在LH峰值后30min内开始细胞浸润，在壁粒层内半部表达LH受体的细胞体比例增加，直到排卵时间，排卵发生在LH峰值后约10h。在此期间，许多最初的瓶状细胞似乎从基板分离出来，获得更圆的形状，具有多个丝状足。LH高峰后约4小时开始，卵泡发生排卵的顶表面壁粒层开始变薄，基底外侧表面出现内陷和收缩。我们的研究结果提出了一个问题，即黄体生成素刺激颗粒细胞侵入是否会导致卵泡结构的这些变化，从而促进排卵。
Luteinizing hormone (LH) induces ovulation by acting on its receptors in the mural granulosa cells that surround a mammalian oocyte in an ovarian follicle. However, much remains unknown about how activation of the LH receptor modifies the structure of the follicle such that the oocyte is released and the follicle remnants are transformed into the corpus luteum. The present study shows that the preovulatory surge of LH stimulates LH receptor-expressing granulosa cells, initially located almost entirely in the outer layers of the mural granulosa, to rapidly extend inwards, intercalating between other cells. The cellular ingression begins within 30 min of the peak of the LH surge, and the proportion of LH receptor-expressing cell bodies in the inner half of the mural granulosa layer increases until the time of ovulation, which occurs at about 10 h after the LH peak. During this time, many of the initially flask-shaped cells appear to detach from the basal lamina, acquiring a rounder shape with multiple filipodia. Starting at about 4 h after the LH peak, the mural granulosa layer at the apical surface of the follicle where ovulation will occur begins to thin, and the basolateral surface develops invaginations and constrictions. Our findings raise the question of whether LH stimulation of granulosa cell ingression may contribute to these changes in the follicular structure that enable ovulation.