Phylogenetic relationships of the G gene sequence of bovine ephemeral fever virus isolated in Japan, Taiwan and Australia
Phylogenetic relationships of the G gene sequence of bovine ephemeral fever virus isolated in Japan, Taiwan and Australia
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DOI：
10.1016/j.vetmic.2009.01.021
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发表时间：
2009-06-12
影响因子：
3.3
通讯作者：
Yamakawa, Makoto
中科院分区：
文献类型：
--
作者：
Kato, Tomoko;Aizawa, Maki;Yamakawa, Makoto
对编码牛短暂热病毒（BEFV）中和抗原的 G 基因进行了表征，以明确该病毒在日本的分子流行病学以及日本、台湾和澳大利亚分离株之间的遗传关系。无论分离年份如何，该基因的核苷酸和氨基酸序列在日本菌株中都高度保守，并且与台湾菌株密切相关。通过系统发育分析，日本和台湾菌株被明确分为三个时间簇：1966年、1984-1989年和1996-2004年，这表明牛短暂性热的流行可能几乎同时发生在同一基因型的国家。另一方面，澳大利亚菌株与这些东亚菌株关系较远，并位于系统发育树的独立第四簇中。表明中和表位中残基224、271和499处的三个氨基酸取代（其中两个产生新的糖基化序列）是牛短暂热病毒的抗原变异的原因。使用在日本分离的牛短暂热病毒进行的交叉中和试验表明，基于最古老的日本毒株 YHL 开发的疫苗似乎仍能有效控制日本的牛短暂热。 (C) 2009 Elsevier B.V. 保留所有权利。
The G gene encoding the neutralization antigen of bovine ephemeral fever virus (BEFV) was characterized in order to define the virus's molecular epidemiology in Japan and the genetic relationships among the Japanese, Taiwanese and Australian isolates. The nucleotide and amino acid sequences of the gene were highly conserved among the Japanese strains, regardless of the year of isolation, and were closely related to the Taiwanese strains. By phylogenetic analysis, the Japanese and Taiwanese strains were classified clearly into three chronological clusters: 1966, 1984-1989 and 1996-2004, indicating that the epidemics of bovine ephemeral fever may occur almost simultaneously in both countries by the same genotype. On the other hand, the Australian strains were distantly related to these East Asian strains and placed in the independent fourth cluster of the phylogenetic tree. It is suggested that three amino acid substitutions at residues 224, 271 and 499 in the neutralizing epitopes, of which two generate new glycosylation sequences, are responsible for antigenic variations of bovine ephemeral fever virus. The cross-neutralization test using the bovine ephemeral fever virus isolated in Japan demonstrated that the vaccine developed based on the oldest Japanese strain, YHL, appears to still be effective for controlling bovine ephemeral fever in Japan. (C) 2009 Elsevier B.V. All rights reserved.