Molecular cloning and characterization of a novel γ-aminobutyrate transaminase cDNA from the Monascus ruber Mr-5
Molecular cloning and characterization of a novel γ-aminobutyrate transaminase cDNA from the Monascus ruber Mr-5
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红曲霉 Mr-5 中新型 γ-氨基丁酸转氨酶 cDNA 的分子克隆和表征
DOI：
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发表时间：
2016
影响因子：
0.2
通讯作者：
Jiang Donghua
中科院分区：
文献类型：
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作者：
Ren Hao;Zhang Ting;Qi Yuping;Jiang Donghua
从富含γ-氨基丁酸（GABA）的红酵母Mr-5中分离到一个编码4-氨基丁酸转氨酶（MrGABA-T）的全长cDNA，该酶催化γ-氨基丁酸（GABA）转化为琥珀酸半醛。MrGABA-T的cDNA全长为1563 bp，开放阅读框（ORF）编码520个氨基酸残基。推导的蛋白质具有9.12的等电点（pI）和计算的分子量为57.5 kDa。系统进化树分析表明，MrGABA-T与曲霉属GABA-Ts的亲缘关系较近。.在本研究中，还通过破坏M.橡胶Mr-5。结果表明，缺失MrGABA-T的菌株（△MrGABA-T）的GABA最大产量约为M. ruber Mr-5（2.67g/L）本研究为在分子水平上提高M. ruber
A full-length cDNA encoding 4-aminobutyrate transaminase (designated as MrGABA-T) which catalyzes the conversion of γ-aminobutyric acid (GABA) to succinic semialdehyde, was isolated from the GABA-rich Monascus ruber Mr-5. The full-length cDNA of MrGABA-T has a 1563 bp open reading frame (ORF) encoding a protein of 520 amino acid residues. The deduced protein has an isoelectric point (pI) of 9.12 and a calculated molecular weight of 57.5 kDa. Phylogenetic tree analysis reveals that MrGABA-T is more closely related to GABA-Ts from Aspergillus than to those of other fungi. . In this study, the function of MrGABA-T, a gene regulating GABA production, was also analyzed by the disruption of MrGABA-T in M. ruber Mr-5. The.results revealed that the GABA maximum production of MrGABA-T-deleted strain (△MrGABA-T) was about 2.8-fold as that of M. ruber Mr-5 (2.67g/L)..This work will make a contribution to enhance GABA production at the molecular level in M. ruber