THE EFFECT OF FOREBRAIN LESIONS IN THE NEONATAL RAT - SURVIVAL OF MIDBRAIN DOPAMINERGIC-NEURONS AND THE CROSSED NIGROSTRIATAL PROJECTION
THE EFFECT OF FOREBRAIN LESIONS IN THE NEONATAL RAT - SURVIVAL OF MIDBRAIN DOPAMINERGIC-NEURONS AND THE CROSSED NIGROSTRIATAL PROJECTION
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DOI：
10.1002/cne.902180105
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发表时间：
1983-01-01
影响因子：
2.5
通讯作者：
REIS, DJ
中科院分区：
文献类型：
--
作者：
JAEGER, CB;JOH, TH;REIS, DJ
将病变置于1日龄大鼠幼仔的纹状体和嗅结节中。对照组和实验组的动物被饲养到成年。用辣根过氧化物酶或FAST Blue逆行示踪剂注射到部分实验动物的未损伤纹状体内，观察中脑神经元的传出投射。用酪氨酸羟化酶免疫细胞化学定位法监测中脑多巴胺能神经元的存活情况。在尾壳核的小病变没有明显的影响，在中脑含酪氨酸羟化酶的神经元的生存，但密度的多巴胺能末梢邻近的病变增加，其余的尾壳核。纹状体病变涉及的估计面积> 1/3导致黑质多巴胺神经元的损失。纹状体的喙部病变主要影响黑质中位于喙部的神经元。尾壳核的背侧病变导致背侧A9神经元的消失。A10和A8多巴胺神经元群的减少发生，如果新生儿病变涉及嗅结节和延髓核。一些含酪氨酸羟化酶的神经元即使在最大的病变后仍然存在。这些多巴胺能神经元形成了一个交叉的黑质纹状体通路，这是证实了逆行运输示踪剂。这种交叉投影在成人的密度似乎不受新生儿病变的影响。新生大鼠多巴胺能神经元与其靶点的拓扑有序投射。这些纤维的重排似乎有限，但轴突终末密度的补偿性增加是明显的，在部分受损的目标领域。
Lesions were placed in the striatum and the olfactory tubercle of 1-day-old rat pups. Control and experimental animals were raised to adulthood. Efferent projections of mesencephalic neurons were examined by injecting the retrograde tracers horseradish peroxidase or FAST Blue into the undamaged striata of some experimental animals. The survival of the mesencephalic dopaminergic neurons was monitored by using immunocytochemical localization of tyrosine hydroxylase. Small lesions in the caudate-putamen had no appreciable effect on the survival of tyrosine hydroxylase-containing neurons in the mesencephalon, but the density of dopaminergic terminals adjacent to the lesion increased in the remaining caudate-putamen. Striatal lesions that involved an estimated area of > 1/3 resulted in loss of dopamine neurons of the substantia nigra compacta. Rostral lesions in the striatum affected mostly rostrally positioned neurons in the substantia nigra. Dorsal lesions of the caudate-putamen resulted in disappearance of dorsal A9 neurons. Reduction of the A10 and A8 dopamine neuron groups occurred if the neonatal lesions involved the olfactory tubercle and nucleus accumbens. Some tyrosine hydroxylase-containing neurons persisted even after the largest lesions. These dopaminergic neurons formed a crossed nigrostriatal pathway which was confirmed by retrogradely transported tracers. The density of this crossed projection in the adult appeared unaffected by the neonatal lesion. Dopaminergic neurons from topographically ordered projections with their targets in the newborn rat. Rearrangement of these fibers appeared limited, but compensatory increase of axon terminal density was evident in partially lesioned target areas.