Effect of exercise intensity and hypoxia on skeletal muscle AMPK signaling and substrate metabolism in humans
Effect of exercise intensity and hypoxia on skeletal muscle AMPK signaling and substrate metabolism in humans
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DOI：
10.1152/ajpendo.00464.2005
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发表时间：
2006-04-01
影响因子：
5.1
通讯作者：
McConell, GK
中科院分区：
文献类型：
--
作者：
Wadley, GD;Lee-Young, RS;McConell, GK
我们在人体骨骼肌中比较了在常氧和低氧条件下绝对与相对运动强度对AMP活化蛋白激酶（AMPK）信号传导和底物代谢的影响。8名未经训练的男性在低氧条件下骑自行车30分钟（11.5% O-2，111 +/- 12 W，72 +/- 3%缺氧（V）超过dotO（2个峰值）; 72%缺氧）或在常氧条件下（20.9% O-2）匹配相同的绝对值（111 +/- 12 W，51 +/- 1%常氧（V）超过点O-2峰; 51%常氧）或相对（相对于点O-2峰上的（V））强度（171 +/- 18 W，点O-2峰上的73 +/- 1%常氧（V）; 73%常氧）。AMPK活性、AMPK α Thr（172）磷酸化、ACC β Ser（221）磷酸化、游离AMP含量和葡萄糖清除率的增加（P < 0.05）受绝对运动强度的影响大于受相对运动强度的影响，在73%常氧时最大，而在51%常氧和72%低氧之间没有差异。与此相反，增加肌糖原的使用，肌肉乳酸含量，血浆中的儿茶酚胺浓度的影响，相对于绝对运动强度，是类似的72%缺氧和73%常氧，这两个试验高于51%常氧。总之，增加肌肉AMPK信号，游离AMP含量，和葡萄糖处置在运动过程中很大程度上是由绝对运动强度，而增加血浆儿茶酚胺水平，肌糖原的使用，和肌肉乳酸水平更密切相关的相对运动强度。
We compared in human skeletal muscle the effect of absolute vs. relative exercise intensity on AMP-activated protein kinase ( AMPK) signaling and substrate metabolism under normoxic and hypoxic conditions. Eight untrained males cycled for 30 min under hypoxic conditions (11.5% O-2, 111 +/- 12 W, 72 +/- 3% hypoxia (V) over dotO(2 peak); 72% Hypoxia) or under normoxic conditions (20.9% O-2) matched to the same absolute (111 +/- 12 W, 51 +/- 1% normoxia (V) over dot O-2 peak; 51% Normoxia) or relative (to (V) over dot O-2 peak) intensity (171 +/- 18 W, 73 +/- 1% normoxia (V) over dot O-2 peak; 73% Normoxia). Increases (P < 0.05) in AMPK activity, AMPK alpha Thr(172) phosphorylation, ACC beta Ser(221) phosphorylation, free AMP content, and glucose clearance were more influenced by the absolute than by the relative exercise intensity, being greatest in 73% Normoxia with no difference between 51% Normoxia and 72% Hypoxia. In contrast to this, increases in muscle glycogen use, muscle lactate content, and plasma catecholamine concentration were more influenced by the relative than by the absolute exercise intensity, being similar in 72% Hypoxia and 73% Normoxia, with both trials higher than in 51% Normoxia. In conclusion, increases in muscle AMPK signaling, free AMP content, and glucose disposal during exercise are largely determined by the absolute exercise intensity, whereas increases in plasma catecholamine levels, muscle glycogen use, and muscle lactate levels are more closely associated with the relative exercise intensity.