Electrochemical enzyme-based blood ATP and lactate sensor for a rapid and straightforward evaluation of illness severity
Electrochemical enzyme-based blood ATP and lactate sensor for a rapid and straightforward evaluation of illness severity
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DOI：
10.1016/j.bios.2021.113832
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发表时间：
2021-11-29
影响因子：
12.6
通讯作者：
Tokeshi, Manabu
中科院分区：
文献类型：
--
作者：
Nishiyama, Keine;Mizukami, Ryohei;Tokeshi, Manabu
本研究旨在开发一种电化学系统，用于以单一格式测量血液ATP和乳酸水平。以前曾报道乳酸与ATP水平的比值可提供替代疾病严重程度评分。虽然严重程度评估对于治疗重症监护病房的急性疾病患者至关重要，但目前没有传感器可以使用相同的检测方法简单快速地测量ATP和乳酸水平。因此，我们构建了一个集成的传感系统，ATP和乳酸使用酶反应和两组电极集成到一个芯片连接到一个由微控制器操作的单个恒电位仪。酶系统涉及腺苷酸激酶、丙酮酸激酶和用于ATP的丙酮酸氧化酶以及用于乳酸的乳酸氧化酶，这两者都产生过氧化氢。多重酶基反应的设计，以尽量减少相应的操作显着没有酶固定到电极上。该系统在存在潜在干扰血液成分（如抗坏血酸盐、丙酮酸盐、ADP、尿酸盐和钾离子）的情况下具有稳健性。使用新传感器成功地测量了血液中的ATP和乳酸水平，具有良好的回收率。使用我们的传感器获得的血液样本分析结果与使用传统方法获得的结果一致。集成基于电极的分析和基于微控制器的系统节省了进一步的操作，使ATP和乳酸水平的直接测量在5分钟内提出的传感器可以作为一个有用的工具，在严重的传染病的管理。
This study aimed to develop an electrochemical system for measuring blood ATP and lactate levels in a single format. The ratio of lactate to ATP levels was previously reported to provide an alternative illness severity score. Although severity evaluation is crucial to treat patients with acute disease admitted to intensive care units, no sensors are currently available to simply and rapidly measure ATP and lactate levels using the same detection method. Therefore, we constructed an integrated sensing system for ATP and lactate using enzymatic reactions and two sets of electrodes integrated into a chip connected to a single potentiostat operated by a microcontroller. The enzymatic system involves adenylate kinase, pyruvate kinase, and pyruvate oxidase for ATP, and lactate oxidase for lactate, both of which produce hydrogen peroxide. Multiplex enzyme-based reactions were designed to minimize the corresponding operations significantly without enzyme immobilization onto the electrodes. The system was robust in the presence of potentially interfering blood components, such as ascorbate, pyruvate, ADP, urate, and potassium ions. The ATP and lactate levels in the blood were successfully measured using the new sensor with good recoveries. The analytical results of blood samples obtained using our sensor were in good agreement with those using conventional methods. Integrating electrode-based analysis and a microcontroller -based system saved further operations, enabling the straightforward measurement of ATP and lactate levels within 5 min. The proposed sensor may serve as a useful tool in the management of serious infectious diseases.