Expression pattern of CYC-like genes relating to a dorsalized actinomorphic flower in Tengia (Gesneriaceae)
Expression pattern of CYC-like genes relating to a dorsalized actinomorphic flower in Tengia (Gesneriaceae)
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苦苣苔科背侧辐射花相关 CYC 样基因的表达模式
DOI：
10.1111/j.1759-6831.2010.00091.x
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发表时间：
2010-09-01
影响因子：
3.7
通讯作者：
Wang, Yin-Zheng
中科院分区：
文献类型：
--
作者：
Pang, Hong-Bo;Sun, Qing-Wen;Wang, Yin-Zheng
天南星科植物天南星被称为苦苣苔科的“天然花”，因为它具有几乎完美的辐射对称花，从第一轮到第三轮。这将是特别有趣的，知道是否或如何CYC类基因的活动与这种类型的完美辐射对称花。为了解决这个问题，我们已经分离出四个CYC样TCP基因，并进行了调查，他们在Tengia的表达模式。TsCYC 1C和TsCYC 1D具有相似的表达模式，在所有五个花瓣和雄蕊中检测到强信号，而TsCYC 2A和TsCYC 2B仅在非常早期的花分生组织中瞬时表达。我们的研究结果表明，TsCYC 1C和TsCYC 1D的表达从背侧到腹侧花瓣的扩展可能是负责完全背侧化的辐射对称花冠的进化形成，即扩展的CYC-like基因背侧身份在藤属花冠的功能域。然而，TsCYC 1C和TsCYC 1D的表达与雄蕊败育无关，因此，TsCYC基因在功能上并不抑制登吉亚花的雄蕊发育。这可能是由于改变了顺式活性，导致细胞周期相关基因从Tengia的TsCYC调控途径中解偶联。
Tengia has been called a "natural peloria" in the family Gesneriaceae because it exhibits an almost perfect actinomorphic flower from whorl one to whorl three. It would be especially interesting to know whether or how CYC-like gene activities are related to this type of perfect actinomorphic flower. To address this, we have isolated four CYC-like TCP genes and carried out an investigation on their expression patterns in Tengia. TsCYC1C and TsCYC1D have similar expression patterns with strong signals being detected in all five petals and stamens, whereas TsCYC2A and TsCYC2B are only transiently expressed in the very early floral meristem. Our results suggest that the expansion of the expressions of TsCYC1C and TsCYC1D from the dorsal to the ventral petals is likely responsible for the evolutionary formation of the fully dorsalized actinomorphic corolla, that is, an expanded functional domain of CYC-like gene dorsal identity in Tengia corolla. However, the expressions of TsCYC1C and TsCYC1D are not correlated with stamen abortion; therefore, TsCYC genes do not functionally repress the stamen development in Tengia flowers. This is probably due to changed cis-activities that result in the cell cycle-related genes uncoupling from the TsCYC regulatory pathway in Tengia.