RELATIONSHIP BETWEEN GINGIVAL CREVICULAR FLUID LEVELS OF ASPARTATE-AMINOTRANSFERASE AND ACTIVE TISSUE DESTRUCTION IN TREATED CHRONIC PERIODONTITIS PATIENTS
RELATIONSHIP BETWEEN GINGIVAL CREVICULAR FLUID LEVELS OF ASPARTATE-AMINOTRANSFERASE AND ACTIVE TISSUE DESTRUCTION IN TREATED CHRONIC PERIODONTITIS PATIENTS
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DOI：
10.1111/j.1600-0765.1990.tb00896.x
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发表时间：
1990-03-01
影响因子：
3.5
通讯作者：
PAGE, RC
中科院分区：
文献类型：
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作者：
PERSSON, GR;DEROUEN, TA;PAGE, RC
来自几个来源的数据表明存在疾病活动性和疾病非活动性牙周袋，并且疾病进展发生在活动的爆发中。目前使用的诊断程序在任何给定的时间点都不能区分疾病活动和疾病非活动部位。我们报告的研究结果，旨在确定是否水平的酶天冬氨酸氨基转移酶（AST）在龈沟液（GCF）与疾病的活动性评估的水平牙龈炎症和探测附着损失。25名先前接受过治疗的牙周炎患者参加了一个季度的回忆维持计划，他们经历了复发性牙周恶化，作为实验对象。每隔3个月对患者进行为期2年的评估。记录菌斑指数、牙龈指数和探测附着水平的值，并从4颗第一磨牙的近颊面和4颗侧门牙的远端采集30秒的牙龈液样本。使用Periotron 6000测量GCF体积，并通过标准方法测量AST活性。根据附着丧失的确定程度以及牙龈炎症的严重程度，将部位分级，并确定这些值与AST水平的关系。三个模型被用来分析得到的数据，都得出了相同的结论。通过附着丧失评估，在确认疾病活动的部位，最大酶水平显着升高，这些部位的最大AST水平平均比其他部位高725个单位（p < 0.0001）。最大AST值也与严重牙龈炎症相关（p < 0.005），其中值比轻度或无牙龈炎症的部位高约600个单位。我们的数据支持这样一种观点，即基于GCF中AST水平的客观诊断测试，区分疾病活动性和疾病非活动性位点是可能的。
Data from several sources demonstrate the disease-active and disease-inactive periodontal pockets exist, and that disease progression occurs in bursts of activity. Currently used diagnostic procedures do not distinguish between disease-active and disease-inactive sites at any given point in time. We report the results of studies aimed at determining whether levels of the enzyme aspartate aminotransferase (AST) in gingival crevicular fluid (GCF) are associated with disease activity as assessed by the level of gingival inflammation and probing attachment loss. 25 previously treated periodontitis patients participating in a quarterly recall maintenance program, who had experienced recurrent periodonal deterioration, served as experimental subjects. Patients were evaluated at 3-month intervals for 2 years. Values for plaque index, gingival index, and probing attachment level were recorded, and 30-second samples of gingival fluid harvested from the mesiobuccal aspect of the 4 first molars and the distal of the 4 lateral incisors. GCF volume was measured using a Periotron 6000, and AST activity was measured by a standard method. Sites were ranked in a hierarchy based on the degree of certainty of attachment loss as well as the severity of gingival inflammation, and the relationship of the values to AST levels was determined. Three models were used to analyze the resulting data, and all led to the same conclusion. Maximum enzyme level was significantly elevated at sites with confirmed disease activity as assessed by attachment loss, with maximum AST levels 725 units higher at these sites, on average, than at other sites (p < 0.0001). Maximum AST values were also associated with severe gingival inflammation (p < 0.005) where values were about 600 units higher than at sites with mild or with no gingival inflammation. Our data support the idea that an ojective diagnostic test, based on levels of AST in GCF, that distinguishes between disease-active and disease-inactive sites may be possible.