Proteomic profiling and identification of immunodominant spore antigens of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis
Proteomic profiling and identification of immunodominant spore antigens of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis
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DOI：
10.1128/aem.00455-06
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发表时间：
2006-09-01
影响因子：
4.4
通讯作者：
Mujer, Cesar V.
中科院分区：
文献类型：
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作者：
DelVecchio, Vito G.;Connolly, Joseph P.;Mujer, Cesar V.
鉴定了蜡样芽孢杆菌属细菌（包括炭疽芽孢杆菌、蜡样芽孢杆菌和苏云金芽孢杆菌）的差异表达和免疫原性孢子蛋白。它们的孢子蛋白的比较蛋白质组学分析将这三个物种彼此区分开，以及从无毒菌株中区分出毒性菌株。通过基质辅助激光解吸电离飞行时间质谱分析，对所有物种的232个开放阅读框编码的458个蛋白质进行了鉴定。许多高度表达的蛋白质，包括延伸因子Tu（EF-Tu）、延伸因子G、60-kDa伴侣蛋白、烯醇化酶、丙酮酸脱氢酶复合物等，在二维凝胶上作为电荷变体存在。这些电荷变体具有相似的质量但具有不同的等电点。大多数已鉴定的蛋白质具有与能量产生、碳水化合物转运和代谢、氨基酸转运和代谢、翻译后修饰和翻译相关的细胞作用。使用B鉴定新的疫苗候选蛋白。炭疽多克隆抗血清。在B中鉴定出15种免疫反应性蛋白。炭疽孢子，而B的免疫反应性蛋白分别为7，14和7。蜡状芽孢杆菌和B的强毒株和无毒毒株。苏云金杆菌孢子。一些免疫显性抗原包括EF-Tu的电荷变体、甘油醛-3-磷酸脱氢酶、二氢硫辛酰胺乙酰转移酶、δ-1-吡咯啉-5-羧酸脱氢酶和二氢硫辛酰胺脱氢酶。丙氨酸消旋酶和中性蛋白酶对B具有独特的免疫原性。炭疽病孢子免疫组的比较分析将对进一步的核酸和免疫检测系统以及下一代疫苗的开发具有重要意义。
Differentially expressed and immunogenic spore proteins of the Bacillus cereus group of bacteria, which includes Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis, were identified. Comparative proteomic profiling of their spore proteins distinguished the three species from each other as well as the virulent from the avirulent strains. A total of 458 proteins encoded by 232 open reading frames were identified by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry analysis for all the species. A number of highly expressed proteins, including elongation factor Tu (EF-Tu), elongation factor G, 60-kDa chaperonin, enolase, pyruvate dehydrogenase complex, and others exist as charge variants on two-dimensional gels. These charge variants have similar masses but different isoelectric points. The majority of identified proteins have cellular roles associated with energy production, carbohydrate transport and metabolism, amino acid transport and metabolism, posttranslational modifications, and translation. Novel vaccine candidate proteins were identified using B. anthracis polyclonal antisera from humans postinfected with cutaneous anthrax. Fifteen immunoreactive proteins were identified in B. anthracis spores, whereas 7, 14, and 7 immunoreactive proteins were identified for B. cereus and in the virulent and avirulent strains of B. thuringiensis spores, respectively. Some of the immunodominant antigens include charge variants of EF-Tu, glyceraldehyde-3-phosphate dehydrogenase, dihydrolipoamide acetyltransferase, Delta-1-pyrroline-5-carboxylate dehydrogenase, and a dihydrolipoamide dehydrogenase. Alanine racemase and neutral protease were uniquely immunogenic to B. anthracis. Comparative analysis of the spore immunome will be of significance for further nucleic acid- and immuno-based detection systems as well as next-generation vaccine development.