Dynamic alterations in early intestinal development, microbiota and metabolome induced by in ovo feeding of L-arginine in a layer chick model
Dynamic alterations in early intestinal development, microbiota and metabolome induced by in ovo feeding of L-arginine in a layer chick model
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DOI：
10.1186/s40104-020-0427-5
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发表时间：
2020-03-10
影响因子：
7
通讯作者：
Wang, Jing
中科院分区：
文献类型：
--
作者：
Dai, Dong;Wu, Shu-geng;Wang, Jing
产前营养对胚胎发育和新生儿生长至关重要，并有可能成为终身健康的主要决定因素。本研究采用蛋鸡模型，研究蛋蛋饲粮中添加l -精氨酸（Arg）对蛋鸡生长、肠道发育、肠道菌群和代谢的影响。治疗包括未注射对照组、注射盐水对照组和含有2、6、10 mg精氨酸的盐水组。结果添加精氨酸可提高雏鸡早期肠道指数和绒毛高度，增加对蛋黄残余脂质的吸收，有利于提高雏鸡早期生长性能。产前添加精氨酸增加了雏鸡早期微生物α -多样性，提高了乳酸菌和梭菌的相对丰度，降低了盲肠变形菌的相对丰度。此外，精氨酸的IOF加速了盲肠菌群组成的变化和潜在益生菌的定植。同时，代谢组学研究表明，在胚胎接受精氨酸IOF后，半乳糖、牛磺酸共轭胆汁酸和脂质的代谢被调节，从而在孵化初期将更多的能量和营养物质用于肠道的快速生长。结论产前添加精氨酸对蛋鸡早期肠道发育、盲肠菌群和宿主代谢有有益影响，有助于提高蛋鸡早期生长性能。这些发现为进一步了解精氨酸的IOF在新孵蛋鸡肠道菌群建立中的作用提供了新的视角，并可以扩展我们对新生儿产前营养、早期细菌定植和肠道发育的基础知识。
Background Prenatal nutrition is crucial for embryonic development and neonatal growth, and has the potential to be a main determinant of life-long health. In the present study, we used a layer chick model to investigate the effects of in ovo feeding (IOF) of L-arginine (Arg) on growth, intestinal development, intestinal microbiota and metabolism. The treatments included the non-injected control, saline-injected control, and saline containing 2, 6, or 10 mg Arg groups. Results IOF Arg increased early intestinal index and villus height, and enhanced uptake of residual yolk lipid, contributing to subsequent improvement in the early growth performance of chicks. Prenatal Arg supplementation also increased the early microbial alpha-diversity, the relative abundance of Lactobacillales and Clostridiales, and decreased the relative abundance of Proteobacteria of cecum in chicks. Furthermore, the shift of cecal microbiota composition and the colonization of potential probiotics were accelerated by IOF of Arg. Simultaneously, metabolomics showed that metabolisms of galactose, taurine-conjugated bile acids and lipids were modulated to direct more energy and nutrients towards rapid growth of intestine at the beginning of post-hatch when embryos received IOF of Arg. Conclusions Prenatal Arg supplementation showed beneficial effects on the early intestinal development, cecal microbiota and host metabolism of layer chicks, contributing to subsequent improvement in the early growth performance. These findings provide new insight into the role of IOF of Arg in the establishment of the gut microbiota of newly-hatched layer chicks, and can expand our fundamental knowledge about prenatal nutrition, early bacterial colonization and intestinal development in neonate.