Elucidating heterogeneous iron biomineralization patterns in a denitrifying As(iii)-oxidizing bacterium: implications for arsenic immobilization.
Elucidating heterogeneous iron biomineralization patterns in a denitrifying As(iii)-oxidizing bacterium: implications for arsenic immobilization.
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阐明异构铁生物矿化模式在一个反硝化As（iii）氧化细菌：砷固定的影响。
DOI：
10.1039/d1en00905b
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发表时间：
2022-03-17
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影响因子：
--
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厌氧硝酸盐依赖的铁（ii）氧化是许多细菌物种共同的过程，它促进铁（iii）矿物质的形成，可以影响土壤和地下水污染物的命运，如砷。在此，我们调查同时硝酸盐依赖的Fe（II）和As（III）的氧化，由Acidovorax sp.菌株ST 3的目的是研究形成的Fe生物矿物，其As固定能力和对细胞的代谢效果。X射线粉末衍射（XRD）和扫描透射电子显微镜（STEM）纳米衍射分别应用于生物矿物表征在散装和纳米级。NanoSIMS（纳米级二次离子质谱法）用于绘制单细胞水平的细胞内和细胞外As和Fe分布，并通过掺入13 C标记的底物（醋酸盐）来追踪代谢活性细胞。细菌细胞之间的代谢异质性检测，与周质铁矿物结壳细胞代谢有害。有趣的是，Fe和As并不共定位在所有的细胞，表明离域网站的As（iii）和Fe（ii）氧化。在XRD中鉴定了Fe（iii）矿物纤铁矿和针铁矿，尽管通过STEM纳米衍射仅鉴定了纤铁矿。细胞外的无定形纳米粒子形成较早，并保留更多的作为（iii/v）比结晶的“薄片”的纤铁矿，这表明较长的孵育期促进形成更多的晶体矿物具有较低的保留能力。因此，此外，硝酸盐促进铁（二）氧化和形成的铁（三）生物矿物质的ST 3细胞保留作为（三/五），虽然这个过程是代谢有害的一些细胞，它值得进一步检查作为一个可行的机制，在缺氧环境中的生物刺激与硝酸盐去除。厌氧硝酸盐依赖的铁（ii）氧化是许多细菌物种共同的过程，它促进铁（iii）矿物质的形成，可以影响土壤和地下水污染物的命运，如砷。
Anaerobic nitrate-dependent iron(ii) oxidation is a process common to many bacterial species, which promotes the formation of Fe(iii) minerals that can influence the fate of soil and groundwater pollutants, such as arsenic. Herein, we investigated simultaneous nitrate-dependent Fe(ii) and As(iii) oxidation by Acidovorax sp. strain ST3 with the aim of studying the Fe biominerals formed, their As immobilization capabilities and the metabolic effect on cells. X-ray powder diffraction (XRD) and scanning transmission electron microscopy (STEM) nanodiffraction were applied for biomineral characterization in bulk and at the nanoscale, respectively. NanoSIMS (nanoscale secondary ion mass spectrometry) was used to map the intra and extracellular As and Fe distribution at the single-cell level and to trace metabolically active cells, by incorporation of a 13C-labeled substrate (acetate). Metabolic heterogeneity among bacterial cells was detected, with periplasmic Fe mineral encrustation deleterious to cell metabolism. Interestingly, Fe and As were not co-localized in all cells, indicating delocalized sites of As(iii) and Fe(ii) oxidation. The Fe(iii) minerals lepidocrocite and goethite were identified in XRD, although only lepidocrocite was identified via STEM nanodiffraction. Extracellular amorphous nanoparticles were formed earlier and retained more As(iii/v) than crystalline “flakes” of lepidocrocite, indicating that longer incubation periods promote the formation of more crystalline minerals with lower As retention capabilities. Thus, the addition of nitrate promotes Fe(ii) oxidation and formation of Fe(iii) biominerals by ST3 cells which retain As(iii/v), and although this process was metabolically detrimental to some cells, it warrants further examination as a viable mechanism for As removal in anoxic environments by biostimulation with nitrate. Anaerobic nitrate-dependent iron(ii) oxidation is a process common to many bacterial species, which promotes the formation of Fe(iii) minerals that can influence the fate of soil and groundwater pollutants, such as arsenic.
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影响因子：
5
作者：
Benzerara, K.;Morin, G.;Brown, G. E., Jr.
通讯作者：
Brown, G. E., Jr.
影响因子：
4.2
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Hegler, Florian;Posth, Nicole R.;Kappler, Andreas
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4.4
作者：
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Coates, JD
影响因子：
64.8
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Lloyd, JR
影响因子：
5
作者：
Hohmann, Claudia;Morin, Guillaume;Kappler, Andreas
通讯作者：
Kappler, Andreas