Evaluation of a Microreactor for Flow Biocatalysis by Combined Theory and Experiment
Evaluation of a Microreactor for Flow Biocatalysis by Combined Theory and Experiment
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DOI：
10.1002/cctc.202000145
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发表时间：
2020-05-07
期刊：
影响因子：
4.5
通讯作者：
Niemeyer, Christof M.
中科院分区：
文献类型：
--
作者：
Burgahn, Teresa;Pietrek, Philip;Niemeyer, Christof M.
微流控装置中酶级联的操作是当前的研究领域，其在生物催化中有多种应用前景。对于流动生物催化系统的优化，期望在计算机中对反应器进行建模，以便能够更好地理解反应系统，从而实现反应系统的经济优化。然而，由于其高度的复杂性，它仍然是很难模拟耦合酶反应。我们在这里描述了一个新的模型，活塞流反应器组成的多孔床的紧凑的均匀颗粒功能化的固定化酮还原酶（Gre2），这是溢出的移动的相含有酶促NADPH辅因子再生系统的基础上葡萄糖脱氢酶（GDH）。通过研究不同的流速，长度和催化床层厚度，我们表明，实验和数学建模的协同作用，可以优化反应系统的时空产率。
The operation of enzyme cascades in microfluidic devices is a current field of research that promises manifold applications in biocatalysis. For an optimization of flow biocatalysis systems it is desirable to model the reactor in silico in order to enable a better understanding and thus an economic optimization of the reaction systems. However, due to their high complexity, it is still difficult to simulate coupled enzyme reactions. We here describe a new model for a plug flow reactor consisting of a porous bed of compact uniform particles functionalized with an immobilized ketoreductase (Gre2) which is overflown by a mobile phase containing the enzymatic NADPH cofactor regeneration system based on glucose dehydrogenase (GDH). By studying different flow rates, lengths and layer thicknesses of the catalytic bed, we show that the synergy of experiment and mathematical modeling can optimize the space-time yields of the reaction system.